Ting Adeline Su Yien, Zoqratt Muhammad Zarul Hanifah Md, Tan Hock Siew, Hermawan Andreas Aditya, Talei Amin, Khu Soon Thiam
School of Science, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor Malaysia.
School of Science, Monash University Malaysia, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor Darul Ehsan Malaysia.
3 Biotech. 2021 Feb;11(2):40. doi: 10.1007/s13205-020-02617-3. Epub 2021 Jan 9.
Microbial communities from a lake and river flowing through a highly dense urbanized township in Malaysia were profiled by sequencing amplicons of the 16S V3-V4 and 18S V9 hypervariable rRNA gene regions via Illumina MiSeq. Results revealed that Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were the dominant prokaryotic phyla; whereas, eukaryotic communities were predominantly of the SAR clade and Opisthokonta. The abundance of and in all sites suggested the possible presence of pathogens in the urban water systems, supported by the most probable number (MPN) values of more than 1600 per 100 mL. Urbanization could have impacted the microbial communities as transient communities (clinical, water-borne and opportunistic pathogens) coexisted with common indigenous aquatic communities (Cyanobacteria). It was concluded that in urban water systems, microbial communities vary in their abundance of microbial phyla detected along the water systems. The influences of urban land use and anthropogenic activities influenced the physicochemical properties and the microbial dynamics in the water systems.
The online version contains supplementary material available at 10.1007/s13205-020-02617-3.
通过Illumina MiSeq对马来西亚一个高度密集城市化城镇中流经的湖泊和河流的微生物群落进行16S V3 - V4和18S V9高变rRNA基因区域扩增子测序分析。结果显示，变形菌门、拟杆菌门、放线菌门和厚壁菌门是主要的原核生物门类；而真核生物群落主要是SAR进化枝和后鞭毛生物。所有位点中 和 的丰度表明城市供水系统中可能存在病原体，每100 mL超过1600的最可能数（MPN）值支持了这一点。城市化可能影响了微生物群落，因为短暂群落（临床、水传播和机会性病原体）与常见的本土水生群落（蓝细菌）共存。得出的结论是，在城市供水系统中，沿水系检测到的微生物门类丰度的微生物群落各不相同。城市土地利用和人为活动的影响改变了供水系统的理化性质和微生物动态。
在线版本包含可在10.1007/s13205 - 020 - 02617 - 3获取的补充材料。
