高效薄层层析法测定胡芦巴种子中薯蓣皂苷元的含量

HPTLC determination of diosgenin in fenugreek seeds.

作者信息

Król-Kogus Barbara, Lamine Khenifi Mohammed, Migas Piotr, Boudjeniba Messaoud, Krauze-Baranowska Mirosława

机构信息

1Department of Pharmacognosy with Medicinal Plants Garden Medical University of Gdańsk 80-416 Gdańsk, Poland.

2Laboratoire d'Ethnobotanique et Substances Naturelles Ecole Normale Supérieure de Kouba B.P 92 Kouba, 16050 Algerie.

出版信息

Acta Pharm. 2018 Mar 1;68(1):97-107. doi: 10.2478/acph-2018-0002.

DOI:10.2478/acph-2018-0002

PMID:29453912

Abstract

A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %.

摘要

在优化了皂苷高效提取和酸水解条件后，建立了一种用于测定胡芦巴种子中薯蓣皂苷元的新型高效薄层色谱-密度测定法。测试了多种方法，其中最佳方法是三步索氏提取，然后用2 mol L-1硫酸水解所得甲醇提取物。在高效薄层色谱Si60F254板上，使用正庚烷/乙酸乙酯（7:3，V/V）混合物和改良茴香醛作为喷雾试剂，可实现薯蓣皂苷元与其他水解产物的最佳分离。该方法经过初步验证，发现波兰和非洲产胡芦巴种子中薯蓣皂苷元的测定含量相似，范围为0.12 - 0.18%。