Chaudhary Spandan A, Chaudhary Pooja S, Syed Bakhtiyar A, Misra Richa, Bagali Prashanth G, Vitalini Sara, Iriti Marcello
Diagnostics and Genomics Research Division, Xcelris Labs Limited, Ahmedabad, Gujarat, India.
Department of Biotechnology, Hemchandracharya North Gujarat University, India.
Acta Sci Pol Technol Aliment. 2018 Oct-Dec;17(4):377-385. doi: 10.17306/J.AFS.0606.
Diosgenin is a very important plant secondary metabolite and raw material for the drug indus- try. Plant sources rich in diosgenin include yam (Dioscorea spp.) and fenugreek (Trigonella foenum-graecum L.). A method for diosgenin extraction from yam extracts has previously been validated, but its extraction from fenugreek plants still requires validation. In addition, all available methods require time-consuming additional purification steps. The present study was aimed at developing a low cost, less time-consuming single-step method for diosgenin extraction from fenugreek.
This study represents a method developed for diosgenin extraction from fenugreek plants without any additional/supportive purification methods such as chromatography or thin-layer chroma- tography. Diosgenin yield estimation and purity analysis by HPLC method, along with accuracy and preci- sion analysis, is presented.
Five different fenugreek varieties were subjected to a newly developed diosgenin extraction method, and an HPLC chromatogram showed a single peak corresponding to diosgenin. Yield was determined by the standard curve method. Limit of detection (LOD) and limit of quantification (LOQ) for the assay were found to be 0.0312 and 0.102 μg, respectively; tcalculated for slope and other statistical parameters were found to be significant (P value < 0.001) for this method.
We have developed a fast, accurate and low cost method for diosgenin extraction from fenu- greek. Although the authors have studied this method only in fenugreek plants, it could be applied to the extraction of a few other plant secondary metabolites, which will help researchers to save time and effort.
薯蓣皂苷元是一种非常重要的植物次生代谢产物和制药工业的原料。富含薯蓣皂苷元的植物来源包括山药（薯蓣属）和胡芦巴（葫芦巴）。此前已验证了从山药提取物中提取薯蓣皂苷元的方法，但从胡芦巴植物中提取仍需验证。此外，所有现有方法都需要耗时的额外纯化步骤。本研究旨在开发一种低成本、耗时少的从胡芦巴中提取薯蓣皂苷元的单步方法。
本研究提出了一种从胡芦巴植物中提取薯蓣皂苷元的方法，无需任何额外的/辅助的纯化方法，如色谱法或薄层色谱法。给出了通过高效液相色谱法进行薯蓣皂苷元产量估算和纯度分析，以及准确性和精密度分析。
对五个不同的胡芦巴品种采用新开发的薯蓣皂苷元提取方法，高效液相色谱图显示对应薯蓣皂苷元的单峰。通过标准曲线法测定产量。该测定方法的检测限（LOD）和定量限（LOQ）分别为0.0312和0.102μg；该方法斜率的计算t值和其他统计参数均具有显著性（P值<0.001）。
我们开发了一种从胡芦巴中快速、准确且低成本提取薯蓣皂苷元的方法。尽管作者仅在胡芦巴植物中研究了该方法，但它可应用于其他几种植物次生代谢产物的提取，这将有助于研究人员节省时间和精力。
