Department of Hematology, Tongji Hospital, Tongji Medical College, Hua Zhong University of Science and Technology, Wu Han, Hubei, China.
Department of Hematology, Tongji Hospital, Tongji Medical College, Hua Zhong University of Science and Technology, Wu Han, Hubei, China.
Exp Cell Res. 2018 Jun 1;367(1):30-36. doi: 10.1016/j.yexcr.2018.02.010. Epub 2018 Feb 14.
PAX5 mutations have important role in leukemogenesis and leukemia relapse, cancer cell dormancy participates in cancer relapse, but there was no report about PAX5 mutation inducing cancer cell dormancy. we constructed the PAX5 deletion Raji cell lines using gene editing technology, evaluated dormancy biological characteristics of cell lines. Our results showed PAX5 haploinsufficiency restrained the proliferation of Raji cells, induced G0/G1 arrest of Raji cells, reduced chemotherapy sensitivity. The tumor formation rate reduced in PAX5 mutation Raji cells. Our results showed PAX5 insufficiency induced cancer cell dormancy in Raji cell.
PAX5 突变在白血病发生和白血病复发中起重要作用，癌细胞休眠参与癌症复发，但尚无关于 PAX5 突变诱导癌细胞休眠的报道。我们使用基因编辑技术构建了 PAX5 缺失 Raji 细胞系，评估了细胞系的休眠生物学特性。我们的结果表明，PAX5 杂合不足抑制 Raji 细胞的增殖，诱导 Raji 细胞 G0/G1 期阻滞，降低化疗敏感性。PAX5 突变 Raji 细胞的肿瘤形成率降低。我们的结果表明，PAX5 不足诱导 Raji 细胞中的癌细胞休眠。
