Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Department of Horticultural Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Int J Biol Macromol. 2018 Jul 1;113:662-668. doi: 10.1016/j.ijbiomac.2018.02.043. Epub 2018 Feb 15.
The aim of present study was to design a controlled release system using Cuminum cyminum essential oil loaded chitosan nanoparticles (CEO-CSNPs) and evaluate its effect on catalase (CAT), glutathione reductase (GR), peroxidase (POD) activity and ascorbic acid content of Agaricus bisporus fruit bodies during 20days of storage at 4°C. The success of encapsulation was evaluated through TEM, DLS, FT-IR and spectrophotometry and its release behavior was studied in buffer solutions with different pH. The CEO-CSNPs exhibited an average size of 30 to 80nm with a spherical shape. Encapsulation efficiency (EE) and loading capacity (LC) were 4.46 to 17.89% and 2.47 to 6.68%, respectively. The highest CAT and GR activity was observed in samples packed with CEO-CSNPs after 15days of storage. In contrast, POD activity reached a peak at the end of storage in control samples. Interestingly, after 20days the level of POD increased 17.13% in CEO-CSNPs treatment, as compared with the initial level of the mentioned enzyme. At the end of storage, ascorbic acid content in samples treated with CEO-CSNPs was significantly higher than that detected in the control samples. In brief, application of CEO loaded chitosan nanoparticles in packages effectively increased the antioxidant activity in white button mushroom and showed promising results for extending the shelf life of treated samples.
本研究旨在设计一种使用孜然精油负载壳聚糖纳米粒子（CEO-CSNPs）的控释系统，并评估其对 4°C 下贮藏 20 天的双孢蘑菇子实体过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）、过氧化物酶（POD）活性和抗坏血酸含量的影响。通过 TEM、DLS、FT-IR 和分光光度法评估包封的成功，并在不同 pH 值的缓冲溶液中研究其释放行为。CEO-CSNPs 表现出 30 至 80nm 的平均粒径，呈球形。包封效率（EE）和载药量（LC）分别为 4.46%至 17.89%和 2.47%至 6.68%。在贮藏 15 天后，用 CEO-CSNPs 包装的样品中观察到 CAT 和 GR 活性最高。相比之下，在对照样品中，POD 活性在贮藏结束时达到峰值。有趣的是，在 20 天的贮藏期后，与酶的初始水平相比，CEO-CSNPs 处理的 POD 水平增加了 17.13%。在贮藏结束时，用 CEO-CSNPs 处理的样品中的抗坏血酸含量明显高于对照样品。总之，在包装中应用负载 CEO 的壳聚糖纳米粒子可有效提高白蘑菇的抗氧化活性，并有望延长处理样品的货架期。
