Team four of the Second group, Graduate school, Third Military Medical University (Army medical university), Chongqing, 400037, China.
Department of Thoracic Surgery, Xinqiao Hospital, Third Military Medical University (Army medical university), Chongqing, 400037, China.
World J Surg Oncol. 2018 Feb 17;16(1):32. doi: 10.1186/s12957-018-1331-8.
SH3-containing guanine nucleotide exchange factor (SGEF), a RhoG-specific guanine nucleotide exchange factor (GEF), was consider as a key signal that determines cancer cell invasion. Although SGEF has been considered to highly express in glioma and prostate cancer. However, it is not well illustrated in LAC.
In this experiment, expression of SGEF was detected in 92 LAC and corresponding normal tissue samples by immunohistochemistry. In addition, we evaluated the invasion and migration of lung adenocarcinoma cells by the gain and loss of SGEF expression. Furthermore, RhoG activity was measured by GST pull-down assay.
SGEF is highly expressed in LAC tissues than in normal lung tissues and was associated with the TNM stage. Lung adenocarcinoma patients with low SGEF subgroup had longer overall survival compared to those with high expression. Furthermore, univariate analysis showed that SGEF expression was an independent prognostic factor for overall survival in lung adenocarcinoma. Silencing of SGEF effectively suppressed the invasion and migration of human lung adenocarcinoma cells in vitro by inhibiting RhoG activity, and over-expression of SGEF could reverse this phenomena.
SGEF is a novel prognostic target in human lung adenocarcinoma.
含 SH3 结构域的鸟嘌呤核苷酸交换因子（SGEF）是一种 RhoG 特异性鸟嘌呤核苷酸交换因子（GEF），被认为是决定癌细胞侵袭的关键信号。尽管 SGEF 被认为在神经胶质瘤和前列腺癌中高度表达，但在肺腺癌（LAC）中尚未得到很好的阐明。
本实验通过免疫组织化学检测了 92 例 LAC 及相应正常组织样本中 SGEF 的表达。此外，我们通过 SGEF 表达的获得和丧失来评估肺腺癌细胞的侵袭和迁移。进一步通过 GST 下拉实验测定 RhoG 活性。
SGEF 在 LAC 组织中的表达明显高于正常肺组织，且与 TNM 分期相关。SGEF 低表达亚组的肺腺癌患者的总生存期长于高表达患者。此外，单因素分析显示，SGEF 表达是肺腺癌患者总生存期的独立预后因素。沉默 SGEF 可有效抑制 RhoG 活性，从而抑制人肺腺癌细胞的体外侵袭和迁移，而过表达 SGEF 可逆转这一现象。
SGEF 是人类肺腺癌的一个新的预后靶标。
