Department of Pharmacy, Indira Gandhi National Tribal University, Amarkantak, Madhya Pradesh, India 484887; SLT Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, India 495009.
SLT Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, India 495009.
J Pharm Sci. 2018 Jun;107(6):1701-1712. doi: 10.1016/j.xphs.2018.02.007. Epub 2018 Feb 15.
Pulmonary vaccination is one of the most promising routes for immunization owing to its noninvasive nature and induction of strong mucosal immunity and systemic response. In the present study, recombinant hepatitis B surface antigen loaded solid fat nanoemulsions (SFNs) as carrier system and monophosphoryl lipid A as an adjuvant-carrier system was prepared and evaluated as multiadjuvanted vaccine system for deep pulmonary vaccination. Deposition and clearance from the deep lung of rats were determined by gamma scintigraphy. Biodistribution of SFNs was determined by the live animal imaging system. SFNs dispersion showed slower clearance as compared with sodium pertechnetate control solution (p <0.001) from the pulmonary region due to the virtue of particulate and hydrophobic nature of formulations. Humoral (sIgA and IgG) and cellular (IL-2 and IF-γ) immune responses were found to be significant (p <0.001) when compared with naïve antigen (recombinant surface antigen without any excipient) solution. Data indicate that deep pulmonary immunization offers a stronger immune response with balanced humoral, mucosal, and cellular immunization, which further needs to be tested in higher animals to support this hypothesis for clinical translation of this so far neglected yet potential target tissue for immunization.
肺部接种是最有前途的免疫接种途径之一，因为它具有非侵入性，可诱导强烈的黏膜免疫和全身反应。在本研究中，制备了负载重组乙型肝炎表面抗原的固体脂肪纳米乳液（SFN）作为载体系统和单磷脂质 A 作为佐剂-载体系统，并将其作为深部肺部接种的多佐剂疫苗系统进行了评估。通过γ闪烁成像术测定大鼠深部肺部的沉积和清除率。通过活体动物成像系统确定 SFN 的分布。SFN 分散体由于颗粒状和疏水性的特性，与高锝酸钠对照溶液相比，在肺部区域的清除速度较慢（p <0.001）。与天然抗原（无任何赋形剂的重组表面抗原）溶液相比，体液（sIgA 和 IgG）和细胞（IL-2 和 IF-γ）免疫应答明显增强（p <0.001）。数据表明，深部肺部免疫接种可提供更强的免疫应答，具有平衡的体液、黏膜和细胞免疫应答，这需要在更高的动物中进行测试，以支持这一假设，即这一迄今为止被忽视但具有潜在免疫接种靶组织的目标组织的临床转化。
