Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital, China Medical University, Shenyang 110001, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, 79 Qingchun Street, Hangzhou 310003, China.
Key Laboratory of AIDS Immunology of National Health and Family Planning Commission, Department of Laboratory Medicine, The First Affiliated Hospital, China Medical University, Shenyang 110001, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, 79 Qingchun Street, Hangzhou 310003, China.
Cell Immunol. 2018 May;327:47-53. doi: 10.1016/j.cellimm.2018.02.004. Epub 2018 Feb 10.
The profound deficiency of Th17 cells contributes to HIV disease progression. The mechanisms of their perturbation remain unclear. Recently, CCR6CD95CD4 naïve T cells (CCR6CD95CD4 T), identified as pre-committed Th17 precursors, were recognized as a subpopulation of CD4 T cells with stem cell properties. Following phenotypical identification, we evaluated their level in patients during chronic HIV infection and following antiretroviral therapy (ART) using flow cytometry. The levels of CCR6CD95CD4 T were decreased during chronic HIV infection and correlated with CD4 T cell counts. Immunological responders harbored higher frequency of CCR6CD95CD4 T, which was associated with CD4/CD8 T cell ratio. Immunological non-responders with lower frequency of CCR6CD95CD4 T failed to exhibit a correlation between CCR6CD95CD4 T and CCR6CD95CD4 T, and displayed elevated ratio of CCR6CD95CD4 T/T. The number of CCR6CD95CD4 T was increased following early ART. These findings shed light on the importance of targeting pre-committed Th17 precursors that enhance immune reconstitution.
Th17 细胞的严重缺乏导致 HIV 疾病进展。其失调的机制仍不清楚。最近，CCR6CD95CD4naive T 细胞（CCR6CD95CD4 T）被鉴定为具有干细胞特性的 Th17 前体的预定向细胞亚群。在表型鉴定后，我们使用流式细胞术评估了慢性 HIV 感染期间和抗逆转录病毒治疗 (ART) 后患者中的这些细胞水平。在慢性 HIV 感染期间，CCR6CD95CD4 T 的水平降低，与 CD4 T 细胞计数相关。免疫应答者具有更高频率的 CCR6CD95CD4 T，这与 CD4/CD8 T 细胞比值相关。免疫无应答者 CCR6CD95CD4 T 的频率较低，未能显示 CCR6CD95CD4 T 与 CCR6CD95CD4 T 之间的相关性，并且 CCR6CD95CD4 T/T 的比值升高。早期 ART 后 CCR6CD95CD4 T 的数量增加。这些发现阐明了针对增强免疫重建的预定向 Th17 前体的重要性。
