Rajabi Zahra, Yazdekhasti Hossein, Noori Mugahi Seyed Mohammad Hossein, Abbasi Mehdi, Kazemnejad Somaieh, Shirazi Abolfazl, Majidi Masoumeh, Zarnani Amir-Hassan
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA.
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, VA 22908, USA.
Reprod Biol. 2018 Mar;18(1):122-131. doi: 10.1016/j.repbio.2018.02.001. Epub 2018 Feb 15.
Follicle culture provides a condition which can help investigators to evaluate various aspects of ovarian follicle growth and development and impact of different components and supplementations as well as presumably application of follicle culture approach in fertility preservation procedures. Mesenchymal Stem Cells (MSCs), particularly those isolated from menstrual blood has the potential to be used as a tool for improvement of fertility. In the current study, a 3D co-culture system with mice preantral follicles and human Menstrual Blood Mesenchymal Stem Cells (MenSCs) using either collagen or alginate beads was designed to investigate whether this system allows better preantral follicles growth and development. Results showed that MenSCs increase the indices of follicular growth including survival rate, diameter, and antrum formation as well as the rate of in vitro maturation (IVM) in both collagen and alginates beads. Although statistically not significant, alginate was found to be superior in terms of supporting survival rate and antrum formation. Hormone assay demonstrated that the amount of secreted 17 β-estradiol and progesterone in both 3D systems increased dramatically after 12 days, with the highest levels in system employing MenSCs. Data also demonstrated that relative expression of studied genes increased for Bmp15 and Gdf9 and decreased for Mater when follicles were cultured in the presence of MenSCs. Collectively, results of the present study showed that MenSCs could improve indices of follicular growth and maturation in vitro. Further studies are needed before a clinical application of MenSCs-induced IVM is considered.
卵泡培养提供了一种条件，有助于研究人员评估卵巢卵泡生长发育的各个方面、不同成分和补充剂的影响，以及卵泡培养方法在生育力保存程序中的可能应用。间充质干细胞（MSCs），尤其是从月经血中分离出的间充质干细胞，有潜力作为一种改善生育力的工具。在本研究中，设计了一种使用胶原蛋白或藻酸盐珠的小鼠前体卵泡与人月经血间充质干细胞（MenSCs）的三维共培养系统，以研究该系统是否能使前体卵泡更好地生长发育。结果表明，MenSCs在胶原蛋白和藻酸盐珠中均能提高卵泡生长指标，包括存活率、直径、腔形成以及体外成熟（IVM）率。虽然在统计学上不显著，但发现藻酸盐在支持存活率和腔形成方面更具优势。激素检测表明，在12天后，两种三维系统中分泌的17β-雌二醇和孕酮量均显著增加，在使用MenSCs的系统中水平最高。数据还表明，当卵泡在MenSCs存在下培养时
