Biotechnology Laboratory, Environmental Health Science and Research Bureau, Healthy Environments and Consumer Safety Branch, Health Canada, Ottawa, K1A 0K9, Canada.
Scientific Services Division, Bureau of Chemical Safety, Food Directorate, Health Products and Food Branch, Health Canada, Ottawa, K1A 0K9, Canada.
Food Chem Toxicol. 2018 Jun;116(Pt A):32-41. doi: 10.1016/j.fct.2018.02.030. Epub 2018 Feb 15.
Microbial-based cleaning products (MBCPs) contain bacteria and chemical constituents. They are used in consumer applications such as odor reduction, unclogging drains, and surface cleaning. To determine the capacity of a model MBCP to contribute to acute allergic lung inflammation, a two-week repeated exposure regimen was used. Mice were exposed by endotracheal instillation to saline alone, MBCP alone, house dust mites (HDM) alone, or sequentially (i.e., MBCP followed by HDM, HDM followed by MBCP, or HDM + MBCP followed by HDM). Both whole MBCP and acellular MBCP filtrate were investigated, and showed minimal differences in the endpoints examined. HDM exposure caused pulmonary perivascular inflammation, bronchiolar mucous cell metaplasia, elevated bronchoalveolar lavage fluid (BALF) eosinophils, and HDM-specific IgG1. For MBCP, notable changes were associated with sequential exposures. MBCP/HDM caused elevated T2 cytokines in BALF, and elevated neutrophils, eosinophils and IL-5 in peripheral blood. Co-administration of MBCP and HDM followed by HDM resulted in elevated blood and BALF eosinophils and HDM-specific IgE and IgG1. These results demonstrated that acellular MBCP filtrate, and not bacteria within MBCPs, potentiated the acute allergic inflammation to HDM. This methodology could be extended to investigate chronic allergic inflammation and inflammatory potential of other MBCPs and biotechnology products with complex compositions.
基于微生物的清洁产品（MBCP）含有细菌和化学成分。它们被用于减少气味、疏通堵塞的排水管道和表面清洁等消费者应用。为了确定模型 MBCP 对急性过敏性肺炎症的贡献能力，采用了为期两周的重复暴露方案。通过气管内滴注将小鼠暴露于单独的生理盐水、单独的 MBCP、单独的屋尘螨（HDM）或顺序暴露（即 MBCP 后紧接着 HDM、HDM 后紧接着 MBCP 或 HDM+MBCP 后紧接着 HDM）。对整个 MBCP 和非细胞 MBCP 滤液都进行了研究，并且在检查的终点方面显示出最小的差异。HDM 暴露导致肺血管周围炎症、细支气管粘液细胞化生、支气管肺泡灌洗液（BALF）嗜酸性粒细胞升高和 HDM 特异性 IgG1。对于 MBCP，与顺序暴露相关的变化显著。MBCP/HDM 导致 BALF 中的 T2 细胞因子升高，外周血中的中性粒细胞、嗜酸性粒细胞和 IL-5 升高。MBCP 和 HDM 共同给药后紧接着再暴露 HDM 导致血液和 BALF 中的嗜酸性粒细胞以及 HDM 特异性 IgE 和 IgG1 升高。这些结果表明，非细胞 MBCP 滤液而不是 MBCP 中的细菌增强了对 HDM 的急性过敏性炎症。这种方法可以扩展到研究其他 MBCP 和具有复杂成分的生物技术产品的慢性过敏性炎症和炎症潜力。
