白菜中对野油菜黄单胞菌 pv. campestris 病抗性与 p-香豆酸诱导的可溶性和细胞壁结合型酚类代谢物特征的关系。

Characterization of p-Coumaric acid-induced soluble and cell wall-bound phenolic metabolites in relation to disease resistance to Xanthomonas campestris pv. campestris in Chinese cabbage.

机构信息

Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju, 61186, South Korea.

Department of Animal Science, Institute of Agricultural Science and Technology, College of Agriculture & Life Science, Chonnam National University, Gwangju, 61186, South Korea; Biotechnology Research Institute, Chonnam National University, Gwangju, 61186, South Korea.

出版信息

Plant Physiol Biochem. 2018 Apr;125:172-177. doi: 10.1016/j.plaphy.2018.02.012. Epub 2018 Feb 13.

DOI:10.1016/j.plaphy.2018.02.012

PMID:29455090

Abstract

To characterize the p-coumaric acid (pCA)-induced phenolic metabolites in relation to the disease resistance against Xanthomonas campestris pv. campestris (Xcc.), the responses of soluble- and cell wall-bound flavonoid and hydroxycinnamic acids compounds to the pretreatment of pCA or the inhibitor of the 4-coumarate-CoA ligase, 3,4-(methylenedioxy) cinnamic acid (MDCA), following Xcc inoculation were assessed, and the resulting data were interpreted with regard to susceptibility to Xcc in Chinese cabbage (Brassica rapa var. pekinensis). At 12 days post-inoculation (DPI) with Xcc, disease symptom development could be distinguished by necrotic lesions, and characterized by an enhanced lipid peroxidation. Overall, pCA acts as a positive stimulus for an accumulation of hydroxycinnamic acids and flavonoids, while MDCA acts as a negative regulator. Pretreatment with pCA resulted in an accumulation of specific hydroxycinnamic acids, pCA, ferulic acid (FA), and sinapic acid (SiA) in both soluble and cell wall-bound forms in Xcc-inoculated leaves, while MDCA pretreatment decreased accumulation in a dose-dependent manner. Two flavonoid compounds, epigallocatechin (EGC) and epigallocatechin gallate (EGCG), showed a similar response to pCA and MDCA pretreatments. These results indicate that a lower disease symptom development in pCA-pretreated leaves was associated with a higher accumulation of hydroxycinnamic acids and flavonoids, and vice-versa in MDCA- and non-pretreated (control) leaves.

摘要

为了研究 p-香豆酸（pCA）诱导的酚类代谢物与抗野油菜黄单胞菌（Xcc）的关系，评估了 pCA 预处理或 4-香豆酸-CoA 连接酶抑制剂 3,4-（亚甲基二氧基）肉桂酸（MDCA）对 Xcc 接种后可溶性和细胞壁结合的类黄酮和羟基肉桂酸化合物的响应，并根据中国白菜（ Brassica rapa var. pekinensis）对 Xcc 的敏感性对所得数据进行了解释。在接种 Xcc 后 12 天（DPI），可以通过坏死病变来区分疾病症状的发展，并通过增强的脂质过氧化来表征。总的来说，pCA 作为羟基肉桂酸和类黄酮积累的正刺激物，而 MDCA 作为负调节剂。pCA 预处理导致在 Xcc 接种叶片中以可溶性和细胞壁结合形式积累特定的羟基肉桂酸，pCA、阿魏酸（FA）和芥子酸（SiA），而 MDCA 预处理以剂量依赖的方式减少积累。两种类黄酮化合物，表没食子儿茶素（EGC）和表没食子儿茶素没食子酸酯（EGCG），对 pCA 和 MDCA 预处理的反应相似。这些结果表明，pCA 预处理叶片中较低的疾病症状发展与羟基肉桂酸和类黄酮的积累较高有关，而 MDCA 和未预处理（对照）叶片则相反。