Li Dongmei, Yang Zhimin, Liu Xinchun, Song Zhen, Feng Zongyun, He Yang
College of Agronomy, Sichuan Agricultural University, Chengdu 611130, P.R. China.
College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, P.R. China.
Z Naturforsch C J Biosci. 2018 Apr 25;73(5-6):191-197. doi: 10.1515/znc-2017-0154.
As an important plateau cereal crop, hulless barley is the principal food for the Tibetan people in China. ADP-glucose pyrophosphorylase (AGPase) is considered as the key enzyme for starch biosynthesis in plants. In this study, cDNAs encoding the small subunit (SSU I) and large subunit (LSU I) of AGPase were isolated from hulless barley. The results showed that SSU I and LSU I were 1438 and 1786 bp in length with a complete open reading frame (ORF) of 1419 and 1572 bp. The ORF-encoded polypeptides of 472 and 523 amino acids were having calculated molecular masses of 52.01 and 58.23 kDa, and the pI values were 5.59 and 6.30. In addition, phylogenetic analysis showed that SSU I and LSU I had the same phylogenetic trends with some species. Furthermore, expression levels in different growth periods and tissues of two hulless barley varieties were analyzed by quantitative reverse transcription-polymerase chain reaction. Gene expression levels of SSU I and LSU I were consistent with the total starch accumulation rate in endosperm. In conclusion, our data confirmed that SSU I and LSU I played an important role in hulless barley starch synthesis.
青稞作为一种重要的高原谷类作物，是中国藏族人民的主要食物。ADP - 葡萄糖焦磷酸化酶（AGPase）被认为是植物淀粉生物合成的关键酶。在本研究中，从青稞中分离出了编码AGPase小亚基（SSU I）和大亚基（LSU I）的cDNA。结果表明，SSU I和LSU I的长度分别为1438和1786 bp，完整开放阅读框（ORF）分别为1419和1572 bp。ORF编码的多肽分别含有472和523个氨基酸，计算分子量分别为52.01和58.23 kDa，pI值分别为5.59和6.30。此外，系统发育分析表明，SSU I和LSU I与某些物种具有相同的系统发育趋势。此外，通过定量逆转录 - 聚合酶链反应分析了两个青稞品种在不同生长时期和组织中的表达水平。SSU I和LSU I的基因表达水平与胚乳中总淀粉积累速率一致。总之，我们的数据证实了SSU I和LSU I在青稞淀粉合成中发挥了重要作用。
