Gao Ting, Shi Xian-Yang
School of Resources and Environmental Engineering, Anhui University, Hefei, 230601, China.
Arch Microbiol. 2018 Aug;200(6):869-876. doi: 10.1007/s00203-018-1493-3. Epub 2018 Feb 17.
The present study investigated the endophytic bacterial communities in the seeds of mature, natural common reed (Phragmites australis) and narrowleaf cattail (Typha angustifolia L.). Additionally, seed endophytic bacterial communities were compared with rhizospheric and root endophytic bacterial communities using Illumina-based sequencing. Seed endophytic bacterial communities were dominated by Proteobacteria (reed, 41.24%; cattail, 45.51%), followed by Bacteroidetes (reed, 12.01%; cattail, 10.41%), Planctomycetes (reed, 10.36%; cattail, 9.09%), Chloroflexi (reed, 8.72%; cattail, 6.45%), Thermotogae (reed, 5.43%; cattail, 6.11%), Tenericutes (reed, 3.63%; cattail, 3.97%) and Spirochaetes (reed, 3.32%; cattail, 3.90%). The dominant genera were Desulfobacter (reed, 8.02%; cattail, 8.96%), Geobacter (reed, 2.74%; cattail, 2.81%), Thiobacillus (reed, 2.71%; cattail, 2.41%), Sulfurimonas (reed, 2.47%; cattail, 2.31%), Methyloversatilis (reed, 2.29%; cattail, 2.05%) and Dechloromonas (reed, 1.13%; cattail, 1.48%). Obvious distinctions were observed among the respective rhizospheric, root endophytic and seed endophytic bacterial communities. Principal coordinate analysis with weighted UniFrac distance and the heat map analysis demonstrated that the seed endophytic bacterial communities were distinct assemblages rather than a subgroup of rhizobacterial communities or root endophytic bacterial communities. These results provide new information regarding endophytic bacteria associated with seeds of wetland plants and demonstrate a variety of genera that have a strong potential to enhance phytoremediation in the wetland ecosystem.
本研究调查了成熟天然芦苇（Phragmites australis）和狭叶香蒲（Typha angustifolia L.）种子中的内生细菌群落。此外，利用基于Illumina的测序技术，将种子内生细菌群落与根际和根内生细菌群落进行了比较。种子内生细菌群落以变形菌门为主（芦苇，41.24%；香蒲，45.51%），其次是拟杆菌门（芦苇，12.01%；香蒲，10.41%）、浮霉菌门（芦苇，10.36%；香蒲，9.09%）、绿弯菌门（芦苇，8.72%；香蒲，6.45%）、栖热袍菌门（芦苇，5.43%；香蒲，6.11%）、柔膜菌门（芦苇，3.63%；香蒲，3.97%）和螺旋体门（芦苇，3.32%；香蒲，3.90%）。优势属为脱硫杆菌属（芦苇，8.02%；香蒲，8.96%）、地杆菌属（芦苇，2.74%；香蒲，2.81%）、硫杆菌属（芦苇，2.71%；香蒲，2.41%）、硫单胞菌属（芦苇，2.47%；香蒲，2.31%）、甲基多功能菌属（芦苇，2.29%；香蒲，2.05%）和脱氯单胞菌属（芦苇，1.13%；香蒲，1.48%）。在各自的根际、根内生和种子内生细菌群落之间观察到明显差异。基于加权UniFrac距离的主坐标分析和热图分析表明，种子内生细菌群落是不同的组合，而不是根际细菌群落或根内生细菌群落的一个亚组。这些结果提供了与湿地植物种子相关的内生细菌的新信息，并证明了多种具有增强湿地生态系统植物修复潜力的属。
