用于红外基质辅助激光解吸/电离质谱成像的新型小型化脉冲模式红外激光系统的表征
Characterization of a novel miniaturized burst-mode infrared laser system for IR-MALDESI mass spectrometry imaging.
作者信息
Ekelöf Måns, Manni Jeffrey, Nazari Milad, Bokhart Mark, Muddiman David C
机构信息
FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University, Raleigh, NC, 27695, USA.
JGM Associates, Inc., 15 District Ave, Burlington, MA, 01803, USA.
出版信息
Anal Bioanal Chem. 2018 Mar;410(9):2395-2402. doi: 10.1007/s00216-018-0918-9. Epub 2018 Feb 17.
Abstract
Laser systems are widely used in mass spectrometry as sample probes and ionization sources. Mid-infrared lasers are particularly suitable for analysis of high water content samples such as animal and plant tissues, using water as a resonantly excited sacrificial matrix. Commercially available mid-IR lasers have historically been bulky and expensive due to cooling requirements. This work presents a novel air-cooled miniature mid-IR laser with adjustable burst-mode output and details an evaluation of its performance for mass spectrometry imaging. The miniature laser was found capable of generating sufficient energy for complete ablation of animal tissue in the context of an IR-MALDESI experiment with exogenously added ice matrix, yielding several hundred confident metabolite identifications. Graphical abstract The use of a novel miniature 2.94 μm burst-mode laser in IR-MALDESI allows for rapid and sensitive mass spectrometry imaging of a whole mouse.
摘要
激光系统作为样品探针和离子源在质谱分析中被广泛应用。中红外激光特别适用于分析高含水量样品，如动植物组织，它利用水作为共振激发的牺牲基质。由于冷却需求，传统的商用中红外激光一直体积庞大且价格昂贵。这项工作展示了一种新型的风冷微型中红外激光，其具有可调的脉冲模式输出，并详细评估了其在质谱成像方面的性能。在添加了外源冰基质的红外基质辅助激光解吸电喷雾电离（IR-MALDESI）实验中，发现该微型激光能够产生足够的能量来完全消融动物组织，从而实现数百种可靠的代谢物鉴定。图形摘要：在IR-MALDESI中使用新型微型2.94μm脉冲模式激光可实现对整个小鼠的快速灵敏质谱成像。
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