Pan Peng, Yang Bai-Xia, Ge Xiao-Lin
Department of Radiotherapy, Huai'an First People's Hospital, Huai'an, Jiangsu 223300, P.R. China.
Department of Radiotherapy, Nantong Tumor Hospital, Nantong, Jiangsu 226000, P.R. China.
Oncol Lett. 2018 Mar;15(3):3870-3875. doi: 10.3892/ol.2018.7779. Epub 2018 Jan 12.
oil emulsion (BJOE) has been used clinically to treat esophageal cancer combined with radiotherapy for numerous years in China. However, the detailed mechanism remains unclear. Thus, the effects of BJOE on the radiosensitivity of esophageal squamous cell carcinoma (ESCC) were evaluated and . The growth inhibitory effects of different BJOE concentrations were determined through an MTT assay. Radiosensitivity was evaluated through focal formation measurements and clone formation assays. The effects of BJOE on radiation-induced apoptosis were examined through flow cytometric analysis. The effects of BJOE on hypoxia-inducible factor 1α (HIF-1α) protein levels and were respectively analyzed through western blot analyses and enzyme-linked immunosorbent assays. BJOE significantly inhibited ECA109 cell proliferation in a dose- and time-dependent manner. Pretreatment with 2.5 mg/ml BJOE increased ECA109 radiosensitivity. BJOE in combination with radiation increased the DNA double-strand breaks. Compared with radiation alone, BJOE and radiation significantly increased the apoptotic rate of ECA109 cells. BJOE also decreased the HIF-1α protein levels and . The results from the present study demonstrated that BJOE enhanced the radiosensitivity of human ESCC. This finding was associated with the inhibition of HIF-1α expression. Therefore, BJOE may be a potential radiotherapy sensitization drug due to its significant anti-hypoxic activity.
在中国，多年来油乳剂（BJOE）一直被临床用于联合放射治疗食管癌。然而，其具体机制仍不清楚。因此，评估了BJOE对食管鳞状细胞癌（ESCC）放射敏感性的影响。通过MTT法测定不同浓度BJOE的生长抑制作用。通过集落形成测量和克隆形成试验评估放射敏感性。通过流式细胞术分析检测BJOE对辐射诱导凋亡的影响。分别通过蛋白质印迹分析和酶联免疫吸附测定分析BJOE对缺氧诱导因子1α（HIF-1α）蛋白水平的影响。BJOE以剂量和时间依赖性方式显著抑制ECA109细胞增殖。用2.5mg/ml BJOE预处理可提高ECA109的放射敏感性。BJOE与辐射联合增加了DNA双链断裂。与单独辐射相比，BJOE和辐射显著提高了ECA109细胞的凋亡率。BJOE还降低了HIF-1α蛋白水平。本研究结果表明，BJOE增强了人ESCC的放射敏感性。这一发现与HIF-1α表达的抑制有关。因此，由于其显著的抗缺氧活性，BJOE可能是一种潜在的放射治疗增敏药物。
