Wu Lian-Guo, Wu Feng-Qing, Chen Hua
Department of Orthopaedics, the Third Affiliated Hospital of Zhejiang University of Chinese Medical, Hangzhou 310005, Zhejiang, China;
Zhongguo Gu Shang. 2017 Dec 25;30(12):1107-1112. doi: 10.3969/j.issn.1003-0034.2017.12.007.
To observe inhibitory effect of zoledronic acid on polyethylene particle-induced periprosthetic osteolysis model.
Thirty male adult specific-pathogen-free SD rats (weighted 250 to 300 g) were randomly divided into sham group, model group, and zoledronic acid group, 10 in each group. Modeling were building by titanium screws and polyethylene particles implanted into right femur of rats, sham group and model group were performed hypodermic injection by 0.9% saline with 2 ml/kg, zoledronic acid with 0.1 ml/mg were injected into zoledronic acid group, once a week for 8 weeks. After 8 weeks, right femur specimens were drawn and used to san microstructure of femoral cancellous bone in rats model was examined by Micro-CT, and the images were treated with three-dimension reconstruction and analysis software BMD, BV/TV, Tb.N, Tb.Th, SMI, BS/BV, Tb.Sp and Tb.Pf and other parameters include.
According to Micro-CT three-dimensional imaging, BMD in model group was significantly decreased than sham group, bone microstructure damage was serious, bone trabecula changed thinning continuity; while bone microstructure was obviously improved compared with model group and zoledronic acid group. After analyzing Micro-CT parameters of femur microstructure, BMD in model group(0.081±0.020) was significantly decreased than control group(0.160±0.018) and zoledronic acid group(0.125±0.012); BV/TV in model group (10.563±1.070) was obviously lower than control group(27.935±1.834) and zoledronic acid group(14.559±1.258); Tb.N in model group (1.005±0.165) was lower than control group(2.058±0.108) and zoledronic acid group(1.515±0.126); while Tb.Th in model group (0.075±0.016) was decreased than control group(0.158±0.016) and zoledronic acid group(0.124±0.011). Meanwhile, SMI in model group(1.817±0.127) was significantly higher than control group(1.104±0.120) and zoledronic acid group(1.547±0.122); BS/BV in model group(35.784±1.650) was higher than control group(21.506±2.771) and zoledronic acid group(30.399±2.730); Tb.Sp in model group(0.735±0.107) was higher than control group(0.423±0.057) and zoledronic acid group(0.577±0.082), TB.Pf in model group(9.088±1.283) was higher than control group(2.447±0.703) and zoledronic acid group(5.862±1.042).
Zoledronic acid could change bone microstructure of rats to inhibit polyethylene particle-induced bone solution, which provides a scientific basis for prevent bone solution by zoledronic acid as a therapeutic intervention.
观察唑来膦酸对聚乙烯颗粒诱导的假体周围骨溶解模型的抑制作用。
将30只体重250至300 g的成年雄性无特定病原体SD大鼠随机分为假手术组、模型组和唑来膦酸组，每组10只。通过将钛螺钉和聚乙烯颗粒植入大鼠右股骨建立模型，假手术组和模型组皮下注射2 ml/kg的0.9%生理盐水，唑来膦酸组注射0.1 ml/mg的唑来膦酸，每周1次，共8周。8周后，取右股骨标本，用Micro-CT检测大鼠模型股骨松质骨的微观结构，并用三维重建和分析软件处理图像，分析骨密度（BMD）、骨体积分数（BV/TV）、骨小梁数量（Tb.N）、骨小梁厚度（Tb.Th）、结构模型指数（SMI）、骨表面积与骨体积比（BS/BV）、骨小梁间距（Tb.Sp）和骨小梁分离度（Tb.Pf）等参数。
根据Micro-CT三维成像，模型组的BMD明显低于假手术组，骨微观结构破坏严重，骨小梁变薄且连续性改变；而唑来膦酸组与模型组相比，骨微观结构明显改善。分析股骨微观结构的Micro-CT参数后发现，模型组的BMD（0.081±0.020）明显低于对照组（0.160±0.018）和唑来膦酸组（0.125±0.012）；模型组的BV/TV（10.563±1.070）明显低于对照组（27.935±1.834）和唑来膦酸组（14.559±1.258）；模型组的Tb.N（1.005±0.165）低于对照组（2.058±0.108）和唑来膦酸组（1.515±0.126）；而模型组的Tb.Th（0.075±0.016）低于对照组（0.158±0.016）和唑来膦酸组（0.124±0.011）。同时，模型组的SMI（1.817±0.127）明显高于对照组（1.104±0.120）和唑来膦酸组（1.547±0.122）；模型组的BS/BV（35.784±1.650）高于对照组（21.506±2.771）和唑来膦酸组（30.399±2.730）；模型组的Tb.Sp（0.735±0.107）高于对照组（0.423±0.057）和唑来膦酸组（0.577±0.082），模型组的TB.Pf（9.088±1.283）高于对照组（2.447±0.7
