Fujikawa Takuro, Imamura Shuji, Tokumaru Motoyuki, Ando Takaaki, Gen Yuki, Hyon Suong-Hyu, Kubota Chikara
The United Graduate School of Veterinary Science, Yamaguchi University, 1677-1 Yoshida, Yamaguchi, 753-8515, Japan.
Kagoshima Prefectural Cattle Breeding Development Institute, 2200 Tsukino, So, 899-8212, Japan.
Cryobiology. 2018 Jun;82:159-162. doi: 10.1016/j.cryobiol.2018.02.009. Epub 2018 Feb 17.
In this study, investigating Carboxylated Poly-l-Lysine (CPLL) for its effectiveness as a new cryoprotectant for bovine sperm is aimed. CPLL is an ampholytic polymer compound, has cryoprotective properties similar to those of anti-freeze protein. The cryopreservation medium used for control group consisted of 6.5% (v/v) glycerol, the cryopreservation medium used for experimental group consisted of 3.25% (v/v) glycerol + 0.5% (w/v) CPLL. There was no consequential difference in sperm motility parameter after thawing whereas there was huge distinction for sperm membrane integrity rate (control vs experimental; 49.6 vs 60.7%, P < 0.01). Conception rate of artificial insemination of experimental group was significantly higher than that of control group (79.0% vs 53.1%, P < 0.01). These results suggest CPLL has protected sperm membrane and leads to improve fertility. This is the first report using CPLL for bovine sperm cryopreservation, it is also expected CPLL can be applied to other animal species.
本研究旨在探究羧化聚-L-赖氨酸（CPLL）作为一种新型牛精子冷冻保护剂的有效性。CPLL是一种两性聚合物化合物，具有与抗冻蛋白相似的冷冻保护特性。对照组使用的冷冻保存培养基由6.5%（v/v）甘油组成，实验组使用的冷冻保存培养基由3.25%（v/v）甘油+0.5%（w/v）CPLL组成。解冻后精子活力参数无显著差异，而精子膜完整率有巨大差异（对照组与实验组；49.6%对60.7%，P<0.01）。实验组人工授精的受胎率显著高于对照组（79.0%对53.1%，P<0.01）。这些结果表明CPLL保护了精子膜并提高了生育能力。这是首次使用CPLL进行牛精子冷冻保存的报告，也期望CPLL可应用于其他动物物种。
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