Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No. 1 Wenhai Road, Aoshanwei Town, Qingdao, China.
Laboratory of Pathology and Immunology of Aquatic Animals, KLMME, Ocean University of China, Qingdao 266003, China.
Mol Immunol. 2018 Apr;96:19-27. doi: 10.1016/j.molimm.2018.02.007. Epub 2018 Feb 20.
T and B lymphocytes are closely related to immunization and pathogen infection. Our previous study confirmed the CD3, CD4-1, CD4-2, CD8β T lymphocytes and IgM B lymphocytes presented in the peripheral blood leukocytes (PBLs) of flounder (Paralichthys olivaceus), in this paper, the variations of T and B lymphocytes of flounder after Hirame novirhabdovirus (HIRRV) infection or immunization were investigated. The flounders were injected with live or inactivated HIRRV, then the percentages of T and B lymphocytes in PBLs were analyzed by Flow cytometry (FCM), total antibodies and HIRRV-specific antibodies in serum were detected by Enzyme-linked immunosorbent assay (ELISA), and expression of twelve immune-related genes in the head kidneys were determined using q-PCR. The results showed that the percentages of CD3, CD4-1, CD4-2, CD8β T lymphocytes and IgM B lymphocytes significantly increased in both infection and immunization groups, in infection group they decreased rapidly after the peak and significantly lower than control levels at the end of infection, in immunization group they went down steadily to the control levels at the end of immunization. The total antibodies and HIRRV-specific antibodies increased first and peaked on the 7 day post infection and on the 14 day post immunization, respectively, then gradually decreased to the control levels. Additionally, twelve immune-related genes were up-regulated in both groups. These results demonstrated that the HIRRV induced both humoral and cellular immunity of flounder, the lymphocytes varied more sharply in infection group than those in immunization group and CD8 T lymphocytes responded much more than CD4 T lymphocytes to HIRRV antigen.
T 和 B 淋巴细胞与免疫和病原体感染密切相关。我们之前的研究证实了牙鲆外周血白细胞（PBL）中存在 CD3、CD4-1、CD4-2、CD8β T 淋巴细胞和 IgM B 淋巴细胞，在本研究中，研究了牙鲆感染或免疫后 T 和 B 淋巴细胞的变化。用活的或灭活的牙鲆虹彩病毒（HIRRV）注射牙鲆，然后通过流式细胞术（FCM）分析 PBL 中 T 和 B 淋巴细胞的百分比，通过酶联免疫吸附试验（ELISA）检测血清中的总抗体和 HIRRV 特异性抗体，并用 q-PCR 测定头肾中 12 种免疫相关基因的表达。结果表明，感染和免疫组中 CD3、CD4-1、CD4-2、CD8β T 淋巴细胞和 IgM B 淋巴细胞的百分比均显著增加，在感染组中，它们在峰值后迅速下降，在感染结束时显著低于对照组水平，在免疫组中，它们在免疫结束时稳定下降至对照组水平。总抗体和 HIRRV 特异性抗体分别在感染后第 7 天和免疫后第 14 天先增加并达到峰值，然后逐渐下降至对照组水平。此外，两组中 12 种免疫相关基因均上调。这些结果表明，HIRRV 诱导了牙鲆的体液和细胞免疫，感染组的淋巴细胞变化比免疫组更剧烈，CD8 T 淋巴细胞对 HIRRV 抗原的反应比 CD4 T 淋巴细胞更为敏感。
