France J T, Jackson W, Keelan J
J Steroid Biochem. 1986 Sep;25(3):375-8. doi: 10.1016/0022-4731(86)90250-5.
We describe a method for the measurement of plasma dehydroepiandrosterone sulphate (DHAS) which incorporates a Triton X-100 solubilised preparation of human placental steroid sulphatase as a hydrolysing agent and a direct radioimmunoassay of liberated DHA using a specific antiserum. The hydrolysis procedure is carried out at 50 degrees C for 1 h and an assay run can be completed in 4 h. As determined by the method, plasma concentrations of DHAS in 32 normal adult men (ages 23-58 yr) had a mean value +/- SD of 5.5 +/- 1.89 mumol/l. For 30 normal adult cyclic women (ages 22-35 yr) the mean plasma concentration of DHAS +/- SD was 3.1 +/- 1.35 mumol/l which was significantly lower (P less than 0.01) than found for men. Plasma DHAS concentration were also measured in 50 hirsute female patients. The mean value +/- SD was 5.03 +/- 2.52 mumol/l which was significantly higher (P less than 0.01) than the value for the normal female group. Some 42% of the hirsute patients had DHAS concentrations above the upper 95% probability limit of the normal range for premenopausal women.
我们描述了一种测量血浆硫酸脱氢表雄酮（DHAS）的方法，该方法采用 Triton X - 100 增溶的人胎盘类固醇硫酸酯酶制剂作为水解剂，并使用特异性抗血清对释放的脱氢表雄酮（DHA）进行直接放射免疫测定。水解过程在 50℃下进行 1 小时，一次测定可在 4 小时内完成。用该方法测定，32 名正常成年男性（年龄 23 - 58 岁）的血浆 DHAS 浓度平均值±标准差为 5.5±1.89μmol/L。对于 30 名正常成年周期女性（年龄 22 - 35 岁），血浆 DHAS 平均浓度±标准差为 3.1±1.35μmol/L，显著低于男性（P < 0.01）。还对 50 名多毛女性患者进行了血浆 DHAS 浓度测定。平均值±标准差为 5.03±2.52μmol/L，显著高于正常女性组（P < 0.01）。约 42%的多毛患者的 DHAS 浓度高于绝经前女性正常范围的 95%概率上限。
