Key Laboratory of Crop Genetics and Breeding of Hebei Province, Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050035, China.
Sci Rep. 2018 Feb 19;8(1):3248. doi: 10.1038/s41598-018-21677-3.
Maize rough dwarf disease (MRDD) is a severe viral disease of maize that occurs worldwide, particularly in the summer maize-growing areas in China, resulting in yield losses and quality deterioration in susceptible maize varieties. An effective solution to control MRDD is to use resistance genes to improve the behavior of susceptible genotypes. Here, we employed maize F populations derived from a cross between susceptible line S221 and resistant line K36 for the deep sequencing of the two DNA pools containing extremely resistant and susceptible F individuals, and used traditional linkage analysis to locate the resistance-related genomic region. The results showed that MRDD resistance in K36 was controlled by a single dominant locus, and an associated region was identified within the genomic interval of 68,396,487 bp and 69,523,478 bp on chromosome 6. Two simple sequence repeat (SSR) markers 6F29R29 and 6F34R34 were tightly linked to the MRDD resistance locus. The findings of the present study improve our understanding of the inheritance patterns of MRDD resistance, and should inform MRDD-resistant maize breeding programs.
玉米粗缩病（MRDD）是一种严重的玉米病毒性疾病，在世界范围内发生，特别是在中国夏季玉米种植区，导致感病玉米品种的产量损失和品质下降。控制 MRDD 的有效方法是利用抗性基因来改善感病基因型的行为。在这里，我们利用玉米 F 群体，该群体源自感病品系 S221 和抗性品系 K36 之间的杂交，对包含极抗和易感 F 个体的两个 DNA 池进行深度测序，并使用传统的连锁分析来定位与抗性相关的基因组区域。结果表明，K36 中的 MRDD 抗性由单个显性基因座控制，并在第 6 号染色体上的基因组区间 68,396,487bp 和 69,523,478bp 内确定了一个相关区域。两个简单序列重复（SSR）标记 6F29R29 和 6F34R34 与 MRDD 抗性基因座紧密连锁。本研究的结果提高了我们对 MRDD 抗性遗传模式的理解，应该为 MRDD 抗性玉米的育种计划提供信息。
