Gerecsei László I, Csillag András, Zachar Gergely, Gévai Lőrinc, Simon László, Dobolyi Árpád, Ádám Ágota
Department of Anatomy, Histology and Embryology, Semmelweis University, Budapest, Hungary.
Laboratory of Sensorimotor Adaptation, Semmelweis University, Budapest, Hungary.
Front Neurosci. 2018 Feb 5;12:27. doi: 10.3389/fnins.2018.00027. eCollection 2018.
The member of synthetic cathinone family, methylenedioxypyrovalerone (MDPV), is a frequently used psychoactive drug of abuse. The objective of our study was to determine the effect of MDPV (administered from the 8th to the 14th day of gestation) on the behavior of neonatal and adolescent mice, as well as its effect on maternal care. We measured maternal care (pup retrieval test, nest building), locomotor activity (open field test), and motor coordination (grip strength test) of dams, whereas on pups we examined locomotor activity at postnatal day 7 and day 21 (open field test) and motor coordination on day 21 (grip strength test). On fresh-frozen brain samples of the dams we examined the expression of two important peptides implicated in the regulation of maternal behavior and lactation: tuberoinfundibular peptide 39 (TIP39) mRNA in the thalamic posterior intralaminar complex, and amylin mRNA in the medial preoptic nucleus. We detected decreased birth rate and survival of offspring, and reduced maternal care in the drug-treated animals, whereas there was no difference between the motility of treated and control mothers. Locomotor activity of the pups was increased in the MDPV treated group both at 7 and 21 days of age, while motor coordination was unaffected by MDPV treatment. TIP39 and amylin were detected in their typical location but failed to show a significant difference of expression between the drug-treated and control groups. The results suggest that chronic systemic administration of the cathinone agent MDPV to pregnant mice can reduce birth rate and maternal care, and it also enhances motility (without impairment of motor coordination) of the offspring.
合成卡西酮家族成员亚甲基二氧吡咯戊酮（MDPV）是一种常用的滥用精神活性药物。我们研究的目的是确定MDPV（在妊娠第8天至第14天给药）对新生和青春期小鼠行为的影响，以及对母性行为的影响。我们测量了母鼠的母性行为（幼崽找回试验、筑巢）、运动活动（旷场试验）和运动协调性（握力试验），而对于幼崽，我们检查了出生后第7天和第21天的运动活动（旷场试验）以及第21天的运动协调性（握力试验）。在母鼠的新鲜冷冻脑样本上，我们检测了与母性行为和泌乳调节有关的两种重要肽的表达：丘脑后内侧核复合体中的促甲状腺激素释放激素39（TIP39）mRNA，以及视前内侧核中的胰淀素mRNA。我们发现药物处理组动物的出生率和后代存活率降低，母性行为减少，而处理组和对照组母鼠的运动能力没有差异。MDPV处理组幼崽在7日龄和21日龄时的运动活动均增加，而运动协调性不受MDPV处理的影响。TIP39和胰淀素在其典型位置被检测到，但药物处理组和对照组之间的表达没有显著差异。结果表明，对怀孕小鼠长期全身给予卡西酮类药物MDPV可降低出生率和母性行为，还可增强后代的运动能力（不损害运动协调性）。
