Swellam Menha, Hashim Maha, Mahmoud Magda Sayed, Ramadan Amal, Hassan Naglaa M
Genetic Engineering and Biotechnology Research Division, Biochemistry Department, High Throughput Molecular and Genetic laboratory, Center for Excellences for Advanced Sciences, National Research Centre, El-Bohouth Street, Dokki, Giza, 12622, Egypt.
Department of Biochemistry, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, Giza, Egypt.
Biochem Genet. 2018 Aug;56(4):283-294. doi: 10.1007/s10528-018-9844-y. Epub 2018 Feb 19.
Acute lymphoblastic leukemia (ALL) is a heterogeneous cancer commonly affecting children due to dysregulation of miRNA expression. In the current study, authors investigated the expression profile for miRNA-125b-1 and miRNA-203 among childhood ALL. Blood samples were collected from newly diagnosed childhood ALL and healthy control children. The expression profile for candidate miRNAs was detected using quantitative RT-PCR analysis. Statistical analysis were performed using receiver operating characteristic curve (ROC) to examine the diagnostic efficacy of the two miRNA and their levels among ALL clinicopathological factors and phenotypes. The median expression level for miRNA-125b-1 was significantly high in childhood ALL; while miRNA-203 level was significantly low in childhood ALL as compared to control ones. MiRNA-125-1 reported significant increase in T-ALL as compared to other ALL phenotypes. Median miRNA-203 level was high in T-ALL followed by pre-B-ALL although no significant difference was reported. Clinicopathological factors did not emphasize significance with either detected miRNAs. Using ROC curve the diagnostic efficacy was significant with an area under the curve 0.858 for miRNA-125b-1 (83.72, 100%) and 0.878 for miRNA-203 (97.67, 86.96%). The combination of the two key miRNAs revealed absolute sensitivity (100%). MiRNA-125b-1 and miRNA-203 can be useful molecular markers for diagnosis of ALL. Further studies with large cohort are warranted to validate these results.
急性淋巴细胞白血病（ALL）是一种异质性癌症，常因微小RNA（miRNA）表达失调而影响儿童。在本研究中，作者调查了儿童ALL中miRNA-125b-1和miRNA-203的表达谱。从新诊断的儿童ALL患者和健康对照儿童中采集血样。使用定量逆转录-聚合酶链反应（RT-PCR）分析检测候选miRNA的表达谱。采用受试者工作特征曲线（ROC）进行统计分析，以检验这两种miRNA的诊断效能及其在ALL临床病理因素和表型中的水平。miRNA-125b-1的中位表达水平在儿童ALL中显著升高；而与对照组相比，miRNA-203水平在儿童ALL中显著降低。与其他ALL表型相比，miRNA-125-1在T-ALL中显著升高。T-ALL中miRNA-203的中位水平较高，其次是前B-ALL，尽管未报告显著差异。临床病理因素与所检测的任何一种miRNA均无显著相关性。使用ROC曲线，miRNA-125b-1的诊断效能显著，曲线下面积为0.858（83.72，100%），miRNA-203为0.878（97.67，86.96%）。这两种关键miRNA的联合检测显示出绝对敏感性（100%）。miRNA-125b-1和miRNA-203可能是诊断ALL的有用分子标志物。有必要进行更大样本量的进一步研究以验证这些结果。
