Zaporozhchenko I A, Bryzgunova O E, Lekchnov E A, Osipov I D, Zaripov M M, Yurchenko Yu B, Yarmoschuk S V, Pashkovskaya O A, Rykova E Yu, Zheravin A A, Laktionov P P
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia.
Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, Russia; Meshalkin Siberian Federal Biomedical Research Center, Novosibirsk, Russia.
Biomed Khim. 2018 Jan;64(1):38-45. doi: 10.18097/PBMC20186401038.
Urine of prostate cancer patients contains tumor-specific biopolymers, including protein- and microvesiclesassociated miRNAs that can potentially be used as oncomarkers. Previously we have characterized urine extracellular vesicles and demonstrated diagnostic potential of their miRNA cargo. In this study, we have performed a comparative analysis of the expression of 84 miRNA in paired samples of urine microvesicles and clarified urine from healthy men, patients with benign hyperplasia and cancer of the prostate using miRCURY LNA miRNA qPCR Panels. Subsets of miRNAs with differences in expression between the fractions of the urine were found in all three groups. Two groups of miRNA were identified based on the patterns of their differential expression. They regulate several key signaling pathways associated with prostate cancer development.
前列腺癌患者的尿液中含有肿瘤特异性生物聚合物，包括与蛋白质和微泡相关的微小RNA，这些物质有可能用作肿瘤标志物。此前我们已对尿液细胞外囊泡进行了表征，并证明了其微小RNA含量的诊断潜力。在本研究中，我们使用miRCURY LNA微小RNA qPCR芯片，对健康男性、良性前列腺增生患者和前列腺癌患者的尿液微泡及澄清尿液的配对样本中84种微小RNA的表达进行了比较分析。在所有三组中均发现了尿液各组分间表达存在差异的微小RNA亚组。根据差异表达模式鉴定出两组微小RNA。它们调节与前列腺癌发展相关的几个关键信号通路。
