Woods W E, Tai H H, Tai C, Weckman T, Wood T, Barios H, Blake J W, Tobin T
Am J Vet Res. 1986 Oct;47(10):2180-3.
A radioiodinated analog of fentanyl was synthesized for use with a commercially available radioimmunoassay for fentanyl. The sensitivity of the modified assay was at least 100 times greater than that of the original assay. Using this modified assay, concentrations of fentanyl as low as 1 pg/ml of fentanyl or fentanyl equivalents in equine urine were detected. Doses of fentanyl 100 times smaller than the minimum dose for a pharmacologic effect were detectable and a pharmacologically effective dose of fentanyl was detectable for up to 96 hours or more. The high sensitivity of the assay indicated that large numbers of urine samples (ie, 10 to 20) probably could be pooled and screened simultaneously, which would result in an economical analysis for fentanyl in the urine of horses after a race. Sufentanil and its metabolites also were detectable, using this assay, but at only about 1% of the efficiency at which fentanyl was detectable.
合成了一种放射性碘化芬太尼类似物，用于与市售的芬太尼放射免疫分析方法配合使用。改进后的分析方法的灵敏度比原来的方法至少高100倍。使用这种改进后的分析方法，可检测出马尿中低至1皮克/毫升的芬太尼或芬太尼当量的浓度。比产生药理作用的最小剂量小100倍的芬太尼剂量也可被检测到，并且在长达96小时或更长时间内都可检测到药理有效剂量的芬太尼。该分析方法的高灵敏度表明，大量尿液样本（即10至20个）可能可以合并并同时进行筛查，这将使得对赛后马尿中的芬太尼进行经济分析成为可能。使用该分析方法也可检测到舒芬太尼及其代谢物，但检测效率仅为芬太尼的1%左右。
