Noda S, Schoner W
Biochim Biophys Acta. 1986 Dec 10;884(3):395-401. doi: 10.1016/0304-4165(86)90189-3.
The intracellular distribution of the glycolytic enzymes hexokinase, glyceraldehyde-3-phosphate dehydrogenase, lactate dehydrogenase and the pyruvate kinase isoenzymes type M1 and type M2 within unfertilized hen eggs was studied. Most of glycolytic enzyme activities were found in the yolk fraction; 8-24% of total glycolytic enzyme activities were found in the vitelline membrane fraction. However, the specific activities of these enzymes in the vitelline membrane fraction are 19-72-fold higher (U/mg protein) and 45-178-fold more concentrated (U/g wet weight) than in the yolk fraction. The study of intracellular localization of pyruvate kinase isoenzymes shows that the blastodisc, latebra and vitelline membrane contain only pyruvate kinase type M2, whereas pyruvate kinase types M1 and M2 are found in the egg yolk. The exclusive occurrence of pyruvate kinase type M2 in the blastodisc is consistent with the concept that this isoenzyme is involved in the cell proliferation. The heterogeneous distribution of the glycolytic enzymes hexokinase, glyceraldehyde-3-phosphate dehydrogenase and lactate dehydrogenase, and the heterogeneous localization of the pyruvate kinase isoenzymes types M1 and M2 indicate that glycolysis is distributed heterogeneously within the unfertilized hen egg cell.
研究了未受精鸡蛋中糖酵解酶己糖激酶、甘油醛-3-磷酸脱氢酶、乳酸脱氢酶以及丙酮酸激酶同工酶M1型和M2型的细胞内分布。大部分糖酵解酶活性存在于蛋黄部分；8%-24%的总糖酵解酶活性存在于卵黄膜部分。然而，这些酶在卵黄膜部分的比活性（U/mg蛋白质）比蛋黄部分高19-72倍，浓度（U/g湿重）高45-178倍。对丙酮酸激酶同工酶细胞内定位的研究表明，胚盘、胚珠和卵黄膜仅含有M2型丙酮酸激酶，而蛋黄中同时存在M1型和M2型丙酮酸激酶。胚盘中仅存在M2型丙酮酸激酶这一现象与该同工酶参与细胞增殖的概念相符。糖酵解酶己糖激酶、甘油醛-3-磷酸脱氢酶和乳酸脱氢酶的不均匀分布，以及丙酮酸激酶同工酶M1型和M2型的不均匀定位表明，糖酵解在未受精鸡蛋细胞内分布不均。
