岩雷鸟（Lagopus muta）16个新微卫星位点的开发与评估以及柳雷鸟（L. lagopus）的跨物种扩增
Development and evaluation of 16 new microsatellite loci for the rock ptarmigan (Lagopus muta) and cross-species amplification for the willow grouse (L. lagopus).
作者信息
Costanzi Jean-Marc, Bergan Frode, Sæbø Mona, Jenkins Andrew, Steifetten Øyvind
机构信息
Department of Natural Sciences and Environmental Health, University College of Southeast Norway, Gullbringvegen 36, 3800, Bø i Telemark, Norway.
出版信息
BMC Res Notes. 2018 Feb 20;11(1):147. doi: 10.1186/s13104-018-3249-1.
OBJECTIVE
The genetic markers designed for this study can facilitate future genetic studies on the rock ptarmigan (Lagopus muta). To our knowledge no microsatellite markers have ever been developed specifically for this species before. These new microsatellite markers will be useful for population genetics studies and for future conservation projects.
RESULTS
Using Next Generation Sequencing 6252 potential microsatellite sequences were found. Sixteen nonpalindromic tetranucleotide microsatellites and their respective primers were selected. The markers were tested on both the rock ptarmigan and the willow grouse (L. lagopus). The number of alleles varied between 2 and 18 for the rock ptarmigan, and between 3 and 13 for the willow grouse. Expected heterozygosity was in the range 0.1244-0.8692 and 0.1358-0.8722 for the rock ptarmigan and the willow grouse, respectively.
目的
本研究设计的遗传标记有助于未来对岩雷鸟（Lagopus muta）进行遗传学研究。据我们所知，此前从未专门为该物种开发过微卫星标记。这些新的微卫星标记将对种群遗传学研究和未来的保护项目有用。
结果
利用新一代测序技术发现了6252个潜在的微卫星序列。选择了16个非回文四核苷酸微卫星及其各自的引物。这些标记在岩雷鸟和柳雷鸟（L. lagopus）上都进行了测试。岩雷鸟的等位基因数量在2至18个之间，柳雷鸟的等位基因数量在3至13个之间。岩雷鸟和柳雷鸟的预期杂合度分别在0.1244 - 0.8692和0.1358 - 0.8722范围内。
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