O'Reilly T, Niven D F
Can J Microbiol. 1986 Sep;32(9):733-7. doi: 10.1139/m86-133.
A variety of biologically important pyridine nucleotides and precursors were examined for their capacities to serve as substrates for the synthesis of NAD by cell fractions derived from Haemophilus parasuis and H. pleuropneumoniae. Of the compounds tested, only NMN and nicotinamide riboside were converted to NAD. These reactions required ATP as co-substrate, and fractions from both organisms could also catalyze the ATP-dependent synthesis of NADP from NAD. In the absence of ATP, and depending on the pyridine compound under study, NAD, NMN, nicotinamide riboside, and also nicotinamide, were detected as products of catabolism. It is concluded that these haemophili possess either three-membered pyridine nucleotide cycles or two-membered cycles with synthetic branches originating with nicotinamide riboside. It is also possible that the pyridine nucleotide cycles of both organisms have nonrecycling branches resulting in the "waste" of usable pyridine compound in the form of nicotinamide.
对多种具有生物学重要性的吡啶核苷酸及其前体进行了检测，以考察它们作为副猪嗜血杆菌和胸膜肺炎放线杆菌细胞组分合成NAD的底物的能力。在所测试的化合物中，只有NMN和烟酰胺核糖能转化为NAD。这些反应需要ATP作为共底物，并且来自这两种微生物的组分也能催化由NAD依赖ATP合成NADP。在没有ATP的情况下，根据所研究的吡啶化合物不同，NAD、NMN、烟酰胺核糖以及烟酰胺被检测为分解代谢的产物。得出的结论是，这些嗜血杆菌拥有三元吡啶核苷酸循环或具有源自烟酰胺核糖的合成分支的二元循环。两种微生物的吡啶核苷酸循环也有可能具有非循环分支，导致以烟酰胺形式“浪费”可用的吡啶化合物。
