Stillman B
Cancer Surv. 1986;5(2):389-404.
The adenovirus E1B gene products are required for productive infection of human cells and for complete transformation of rodent cells in cooperation with the E1A gene products. Two major, unrelated polypeptides of 55,000 (55K) and 19,000 (19K) daltons are encoded by the E1B region. The 55K protein is required for efficient DNA replication, late mRNA transport to the cytoplasm and shut-off of cellular mRNA transport in productively infected cells. This protein is required for virus-mediated, but not DNA-mediated, transformation of rodent cells. It appears that the 55K protein does not directly contribute to cell transformation, but influences the oncogenicity of adenoviruses when they are inoculated into newborn hamsters. In contrast, the 19K protein is required for adenovirus induced cellular transformation and oncogenicity and localizes to membranes of the nuclear envelope, cytoplasm and the cell surface in transformed cells. This protein affects the efficiency of virus growth in some, but not all, human cells by regulating the expression of other adenovirus early genes.
腺病毒E1B基因产物对于人类细胞的有效感染以及与E1A基因产物协同作用使啮齿动物细胞完全转化是必需的。E1B区域编码两种主要的、不相关的多肽，分子量分别为55000（55K）和19000（19K）道尔顿。55K蛋白对于有效DNA复制、晚期mRNA转运至细胞质以及在有效感染的细胞中关闭细胞mRNA转运是必需的。这种蛋白对于病毒介导而非DNA介导的啮齿动物细胞转化是必需的。看来55K蛋白并不直接促进细胞转化，但在将腺病毒接种到新生仓鼠中时会影响腺病毒的致癌性。相比之下，19K蛋白对于腺病毒诱导的细胞转化和致癌性是必需的，并且在转化细胞中定位于核膜、细胞质和细胞表面的膜上。这种蛋白通过调节其他腺病毒早期基因的表达来影响在某些但不是所有人类细胞中的病毒生长效率。
