Grygiel-Górniak Bogna, Kaczmarek Elżbieta, Mosor Maria, Przysławski Juliusz, Nowak Jerzy
Department of Rheumatology and Internal Diseases, Poznan University of Medical Sciences, 28 Czerwca Street 135/147, 61545, Poznan, Poland.
Department of Bioinformatics and Computational Biology, Poznan University of Medical Sciences, Poznan, Poland.
J Appl Genet. 2018 May;59(2):169-177. doi: 10.1007/s13353-018-0434-9. Epub 2018 Feb 20.
As ageing and increased body fat are the signs of insulin resistance, we have studied whether the presence of Pro12Ala and C1431T of peroxisome proliferator-activated receptor gamma 2 gene and Trp64Arg of beta 3-adrenergic receptor gene may predispose to the hyperglycaemia development in postmenopausal women, who have never undergone hypoglycaemic treatment. The distributions of selected allele and genotype frequencies were determined by the PCR-RFLP method in normo- and hyperglycaemic, who have never been diagnosed and treated for diabetes mellitus were measured. The amount of body fat and lean body mass (LBM) were assessed by the bioimpedance method and nutritional habits by 7-day dietary recall. There were no differences between the distribution of genotypes and the allele frequencies of the Pro12Ala, C1431T and Trp64Arg polymorphisms in normo- and hyperglycaemic women. Hyperglycaemic women were characterized by visceral obesity, hypertension, higher serum insulin and triglycerides, higher intake of fat and lower consumption of complex carbohydrates and B vitamins. Normoglycaemic women with Pro12Pro polymorphism acquired higher energy from dietary fat (p < 0.0276) and lower energy from carbohydrates (p < 0.0480) than normoglycaemic Ala12 carriers. Subjects with Pro12Pro polymorphism and LBM > 58% of total body mass or with Trp64Trp and normal triglycerides have higher chance of normoglycaemia. Genotyping for Pro12Ala and Trp64Arg polymorphism in postmenopausal women may have the clinical benefit of predicting hyperglycaemia, thereby contributing to the prevention of diabetes mellitus development in the future. However, not only the genetic background but also the dietary habits (intake of fat, carbohydrates and B vitamins) determine the risk of hyperglycaemia.
由于衰老和体脂增加是胰岛素抵抗的迹象，我们研究了过氧化物酶体增殖物激活受体γ2基因的Pro12Ala和C1431T以及β3-肾上腺素能受体基因的Trp64Arg是否可能使从未接受过降糖治疗的绝经后女性易发生高血糖。通过PCR-RFLP方法测定了从未被诊断和治疗过糖尿病的正常血糖和高血糖患者中所选等位基因和基因型频率的分布。通过生物电阻抗法评估体脂和瘦体重（LBM），通过7天饮食回顾评估营养习惯。正常血糖和高血糖女性在Pro12Ala、C1431T和Trp64Arg多态性的基因型分布和等位基因频率上没有差异。高血糖女性的特征是内脏肥胖、高血压、血清胰岛素和甘油三酯较高、脂肪摄入量较高以及复合碳水化合物和B族维生素的消耗量较低。与正常血糖的Ala12携带者相比，具有Pro12Pro多态性的正常血糖女性从膳食脂肪中获取的能量更高（p < 0.0276），从碳水化合物中获取的能量更低（p < 0.0480）。具有Pro12Pro多态性且LBM>总体重58%的受试者或具有Trp64Trp且甘油三酯正常的受试者血糖正常的几率更高。对绝经后女性的Pro12Ala和Trp64Arg多态性进行基因分型可能具有预测高血糖的临床益处，从而有助于未来预防糖尿病的发生。然而，不仅遗传背景，饮食习惯（脂肪、碳水化合物和B族维生素的摄入量）也决定了高血糖的风险。
