Bezerra Nicholas Morais, Moura Gabriela Hémylin Ferreira, de Araújo Hélio Noberto, Bezerra Francisco Silvestre Brilhante, de Paiva Kaliane Alessandra Rodrigues, de Freitas Mendonça Costa Kizzy Millenn, Costa Wirton Peixoto, Medeiros Dayse Ariane Soares, Batista Jael Soares
Center for Agrarian Sciences, Federal Rural of the Semi-Arid University, Street Francisco Mota, 572, CEP 59625-900, Mossoro, Rio Grande do Norte, Brazil.
Vet Res Commun. 2018 Jun;42(2):131-135. doi: 10.1007/s11259-018-9715-3. Epub 2018 Feb 20.
The present work aimed to investigate the presence of T. vivax DNA in the semen of experimentally infected goats. Twelve male goats native to the Brazilian Northeast, adults, were randomly assigned to two experimental groups: the infected group consisting of six goats infected intravenously with 0.5 mL of blood containing approximately 1.25 × 10 trypomastigotes of T. vivax, and a control group composed of six uninfected goats. After the infection, clinical examinations aiming to evaluate rectal temperature, parasitemia and hematocrit were performed. Semen samples were collected from goats by electroejaculation on the 7th, 14th and 21st days post-infection (dpi). The recombinant DNA-encoding gene encoding the L-like-specific gene for T. vivax. The infection was characterized by increased rectal temperature, high parasitemia and significant reduction of hematocrit values. Results for T. vivax DNA detection using TviCatL-PCR were positive in all semen samples from the infected group collected on 7th, 14th and 21st dpi. The presence of T. vivax DNA in 7th dpi suggests the early invasion of the parasite in the reproductive organs. Also, the finding of T. vivax DNA in all periods analyzed may suggest the continued elimination of the parasite in the semen, which may increase the chances of sexual transmission. Thus, T. vivax DNA is recorded for the first time in the semen of infected goats. Thus, these data are of great importance, since the detection of the T. vivax genetic material in the semen may point to the possibility that the parasite may be transmitted through the sexual pathway.
本研究旨在调查实验感染山羊精液中是否存在间日疟原虫DNA。12只原产于巴西东北部的成年雄性山羊被随机分为两个实验组：感染组由6只静脉注射0.5 mL含有约1.25×10个间日疟原虫锥鞭毛体的血液的山羊组成，对照组由6只未感染的山羊组成。感染后，进行旨在评估直肠温度、寄生虫血症和血细胞比容的临床检查。在感染后第7天、14天和21天通过电刺激从山羊采集精液样本。重组DNA编码间日疟原虫的L样特异性基因。感染的特征是直肠温度升高、高寄生虫血症和血细胞比容值显著降低。使用TviCatL-PCR检测间日疟原虫DNA的结果在感染组第7天、14天和21天采集的所有精液样本中均为阳性。在第7天检测到间日疟原虫DNA表明寄生虫早期侵入生殖器官。此外，在所有分析时期都发现间日疟原虫DNA可能表明寄生虫在精液中持续清除，这可能增加性传播的机会。因此，首次在感染山羊的精液中记录到间日疟原虫DNA。因此，这些数据非常重要，因为在精液中检测到间日疟原虫遗传物质可能表明该寄生虫可能通过性途径传播。
