GeneXpert MTB/RIF 与多重 PCR 检测 mpb64 和 IS6110 对胸腔结核的诊断价值比较。
Comparative evaluation of GeneXpert MTB/RIF and multiplex PCR targeting mpb64 and IS6110 for the diagnosis of pleural TB.
机构信息
Centre for Biotechnology, Maharshi Dayanand University (MDU), Rohtak-124001 (Haryana), India.
Department of Biostatistics, All India Institute of Medical Sciences (AIIMS), New Delhi 110029, India.
出版信息
Future Microbiol. 2018 Mar;13:407-413. doi: 10.2217/fmb-2017-0147. Epub 2018 Feb 21.
AIM
Diagnosis of pleural TB poses serious challenges due to paucibacillary nature of specimens and there is an urgent need to devise a reliable diagnostic test.
METHODS
We compared GeneXpert Mycobacterium tuberculosis/rifampin assay and the multiplex PCR (M-PCR) targeting mpb64 (Rv1980c) and IS6110 in pleural fluids (n = 78) of pleural TB patients and non-TB controls.
RESULTS
The sensitivities of 89.6 and 33.3%, and specificities of 96.7 and 100%, were observed with M-PCR and Xpert assay, respectively.
CONCLUSION
M-PCR showed superiority over Xpert assay and may facilitate an efficient diagnosis of pleural TB.
目的
由于标本的菌量少，胸膜结核的诊断存在很大的挑战，因此迫切需要设计一种可靠的诊断试验。
方法
我们比较了 GeneXpert 结核分枝杆菌/利福平检测法和针对 mpb64（Rv1980c）和 IS6110 的多重 PCR（M-PCR）在胸膜结核患者和非结核对照者的胸腔液中的检测效果（n=78）。
结果
M-PCR 的灵敏度分别为 89.6%和 33.3%，特异性分别为 96.7%和 100%。
结论
M-PCR 比 Xpert 检测法具有优势，可能有助于胸膜结核的有效诊断。
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