Int J Gynecol Cancer. 2018 Mar;28(3):472-478. doi: 10.1097/IGC.0000000000001214.
The aim of this study was to investigate the relationship between BRCA1 and mitotic arrest deficiency protein 2 (MAD2) protein expression, as determined by immunohistochemistry, and clinical outcomes in epithelial ovarian carcinoma (EOC).
A tissue microarray consisting of 94 formalin-fixed paraffin-embedded EOC with fully matched clinicopathological data were immunohistochemically stained with anti-BRCA1 and anti-MAD2 antibodies. The cores were scored in a semiquantitative manner evaluating nuclear staining intensity and extent. Coexpression of BRCA1 and MAD2 was evaluated, and patient survival analyses were undertaken.
Coexpression of BRCA1 and MAD2 was assessed in 94 EOC samples, and survival analysis was performed on 65 high-grade serous carcinomas (HGSCs). There was a significant positive correlation between BRCA1 and MAD2 expression in this patient cohort (P < 0.0001). Both low BRCA1 and low MAD2 are independently associated with overall survival because of HGSC. Low coexpression of BRCA1 and MAD2 was also significantly associated with overall survival and was driven by BRCA1 expression.
BRCA1 and MAD2 expressions are strongly correlated in EOC, but BRCA1 expression remains the stronger prognostic factor in HGSC.
本研究旨在探讨上皮性卵巢癌（EOC）中 BRCA1 和有丝分裂检验点蛋白 2（MAD2）蛋白表达与临床结局的关系。
采用免疫组织化学方法，对 94 例福尔马林固定石蜡包埋的 EOC 组织微阵列进行 BRCA1 和 MAD2 抗体染色，对核染色强度和范围进行半定量评分。评估 BRCA1 和 MAD2 的共表达，并进行患者生存分析。
在 94 例 EOC 样本中评估了 BRCA1 和 MAD2 的共表达，并对 65 例高级别浆液性癌（HGSC）进行了生存分析。在该患者队列中，BRCA1 和 MAD2 的表达呈显著正相关（P<0.0001）。BRCA1 和 MAD2 低表达均与 HGSC 的总生存率独立相关，低共表达 BRCA1 和 MAD2 也与总生存率显著相关，其主要由 BRCA1 表达驱动。
BRCA1 和 MAD2 在 EOC 中表达呈强相关性，但在 HGSC 中 BRCA1 表达仍是更强的预后因素。
