Ruivo R, Capitão A, Castro L F C, Santos M M
CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, U. Porto, Terminal de Cruzeiros do Porto de Leixões, Avenue General Norton de Matos S/N, 4450-208, Matosinhos, Portugal.
FCUP - Faculty of Sciences, Department of Biology, U. Porto, Rua do Campo Alegre 1021/1055, 4169-007, Porto, Portugal.
J Fish Biol. 2018 Apr;92(4):1051-1064. doi: 10.1111/jfb.13564. Epub 2018 Feb 21.
The expression pattern of genes coding for enzymes of the retinoic acid (RA) synthetic and degradation pathways was characterized in adult female zebrafish Danio rerio. Females were conditioned until maturation and post-spawn expression dynamics were determined. A striking upregulation of cyp26b1, but not cyp26a1, was observed following egg deposition, decreasing to initial levels during recovery. A similar, yet lower, fluctuation was observed for aldh1a2 and rdh10a, the enzymes participating in the two-step RA biosynthesis cascade. The present work highlights the dynamics of the adult D. rerio oogenesis and uncovers novel, yet elusive, metabolic contributors. Possible compartmentalized roles for the different gene paralogue isoforms are discussed.
在成年雌性斑马鱼（Danio rerio）中，对编码视黄酸（RA）合成和降解途径中酶的基因表达模式进行了表征。将雌性斑马鱼饲养至成熟，并确定产卵后基因的表达动态。产卵后观察到cyp26b1显著上调，但cyp26a1未上调，恢复期间cyp26b1表达降至初始水平。参与两步RA生物合成级联反应的酶aldh1a2和rdh10a也观察到类似但较低的波动。本研究突出了成年斑马鱼卵子发生的动态变化，并揭示了新的、难以捉摸的代谢贡献者。讨论了不同基因旁系同源异构体可能的分区作用。
