Department of Biology - Animal Physiology , Philipps University Marburg , D-35032 Marburg , Germany.
Institut d'Ecologie et des Sciences de l'Environnement de Paris (UMR iEES-Paris) , INRA , Route de Saint-Cyr , 78026 Versailles Cedex , France.
J Proteome Res. 2018 Apr 6;17(4):1397-1414. doi: 10.1021/acs.jproteome.7b00779. Epub 2018 Feb 28.
In many insects, mating induces drastic changes in male and female responses to sex pheromones or host-plant odors. In the male moth Agrotis ipsilon, mating induces a transient inhibition of behavioral and neuronal responses to the female sex pheromone. As neuropeptides and peptide hormones regulate most behavioral processes, we hypothesize that they could be involved in this mating-dependent olfactory plasticity. Here we used next-generation RNA sequencing and a combination of liquid chromatography, matrix assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and direct tissue profiling to analyze the transcriptome and peptidome of different brain compartments in virgin and mated males and females of A. ipsilon. We identified 37 transcripts encoding putative neuropeptide precursors and 54 putative bioactive neuropeptides from 23 neuropeptide precursors (70 sequences in total, 25 neuropeptide precursors) in different areas of the central nervous system including the antennal lobes, the gnathal ganglion, and the corpora cardiaca-corpora allata complex. Comparisons between virgin and mated males and females revealed tissue-specific differences in peptide composition between sexes and according to physiological state. Mated males showed postmating differences in neuropeptide occurrence, which could participate in the mating-induced olfactory plasticity.
在许多昆虫中，交配会引起雌雄对性信息素或寄主植物气味的反应发生剧烈变化。在雄性蛾 Agrotis ipsilon 中，交配会导致其对雌性性信息素的行为和神经元反应短暂抑制。由于神经肽和肽类激素调节着大多数行为过程，我们假设它们可能参与这种交配依赖的嗅觉可塑性。在这里，我们使用下一代 RNA 测序以及液相色谱、基质辅助激光解吸电离飞行时间 (MALDI-TOF) 质谱和直接组织分析的组合，分析了 A. ipsilon 处女雄蛾和雄蛾交配后的不同脑区的转录组和肽组。我们从中央神经系统的不同区域（包括触角叶、颌神经节和心-咽复合体）的 23 个神经肽前体中鉴定出 37 个编码假定神经肽前体的转录本和 54 个假定生物活性神经肽，共计 70 个序列和 25 个神经肽前体。雌雄之间的比较表明，肽组成在不同组织之间存在性别特异性差异，并且根据生理状态存在差异。交配后的雄蛾在神经肽出现方面表现出交配后的差异，这可能参与了交配诱导的嗅觉可塑性。
