Tessier L H, Jallat S, Sauvageot M, Crystal R G, Courtney M
FEBS Lett. 1986 Nov 24;208(2):183-8. doi: 10.1016/0014-5793(86)81014-6.
Analysis of a series of lambda cII::alpha 1-antitrypsin (alpha 1AT) gene fusions of different sizes showed that increased alpha 1AT expression correlated with the stabilisation of a particular computer-predicted RNA secondary structure. Moreover, significant synthesis of unfused alpha 1AT was achieved by reconstruction of this conformation to permit interaction between the upstream region of the ribosome-binding site and the first part of the alpha 1AT coding sequence. This high-level expression was dependent upon certain silent point mutations in the coding sequence, indicating that RNA primary and secondary structure determinants can operate in concert to dictate the efficiency of protein synthesis.
对一系列不同大小的λ cII::α1-抗胰蛋白酶（α1AT）基因融合体进行分析表明，α1AT表达的增加与一种特定的计算机预测RNA二级结构的稳定相关。此外，通过重建这种构象以允许核糖体结合位点的上游区域与α1AT编码序列的第一部分之间相互作用，实现了未融合α1AT的显著合成。这种高水平表达取决于编码序列中的某些沉默点突变，表明RNA一级和二级结构决定因素可以协同作用来决定蛋白质合成的效率。
