Department of Respiratory Medicine, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Department of Respiratory Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
FASEB J. 2018 Jul;32(7):3957-3967. doi: 10.1096/fj.201701237RR. Epub 2018 Feb 21.
Long noncoding RNAs participate in the progression and initiation of non-small cell lung cancer (NSCLC), although the mechanism remains unknown. The lncRNA identified as small nucleolar RNA host gene 1 ( SNHG1) is a novel lncRNA that is increased in multiple human cancers; however, the regulatory mechanism requires further investigation. In this study, we discovered that SNHG1 was markedly up-regulated in NSCLC tissues and cells and that SNHG1 silencing decreased tumor volumes. Moreover, we explored its regulatory mechanism and found that SNHG1 directly bound to microRNA (miRNA)-145-5p, isolating miR-145-5p from its target gene MTDH. Inhibition of SNHG1 suppressed NSCLC cell viability, proliferation, migration, and invasion in vitro, but its effect was rescued by miR-145-5p inhibition. These results demonstrate that SNHG1 contributes to NSCLC progression by modulating the miR-145-5p/ MTDH axis, and it could potentially be a therapeutic target as well as a diagnostic marker.-Lu, Q., Shan, S., Li, Y., Zhu, D., Jin, W., Ren, T. Long noncoding RNA SNHG1 promotes non-small cell lung cancer progression by up-regulating MTDH via sponging miR-145-5p.
长链非编码 RNA 参与非小细胞肺癌（NSCLC）的发生和发展，但其机制尚不清楚。小核仁 RNA 宿主基因 1（SNHG1）是一种新型长链非编码 RNA，在多种人类癌症中表达增加；然而，其调控机制需要进一步研究。在本研究中，我们发现 SNHG1 在 NSCLC 组织和细胞中明显上调，SNHG1 沉默可降低肿瘤体积。此外，我们还探讨了其调控机制，发现 SNHG1 可直接与 microRNA（miRNA）-145-5p 结合，将 miR-145-5p 从其靶基因 MTDH 上隔离。抑制 SNHG1 可抑制 NSCLC 细胞的活力、增殖、迁移和侵袭，但其作用可被 miR-145-5p 抑制所挽救。这些结果表明，SNHG1 通过调节 miR-145-5p/MTDH 轴促进 NSCLC 的进展，它可能是一个治疗靶点和诊断标志物。-卢强，单珊，李颖，朱丹，金伟，任涛。长链非编码 RNA SNHG1 通过海绵吸附 miR-145-5p 上调 MTDH 促进非小细胞肺癌进展。
