He Jian, Xu Jing, Wu Ping, Liao Lingfeng, Quan Huatao, Kang Jing, Tian Yongquan, Tang Yaoyun
Department of Otorhinolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China.
Oncol Lett. 2018 Mar;15(3):3562-3565. doi: 10.3892/ol.2018.7729. Epub 2018 Jan 4.
This study explored the 605 nm carboxyl of water-soluble quantum dots to assess the practicability of the nasopharyngeal carcinoma marker EBNA1 antibody. We used 605 nm carboxyl water-soluble quantum dots and nasopharyngeal carcinoma EBNA1 antigen in 1-(3-dimethylaminopropyl-3-2-ethylcarbonimine hydrochloride to generate quantum dot-labeled antigen. Gel imaging system showed that serum group and 60 patients with nasopharyngeal carcinoma had significant differences in 30 cases of normal adult serogroup brightness, Levene test in the two groups ELISA absorbance value was P<0.001, namely the nasopharyngeal carcinoma group and the normal adult serum group measured absorbance value difference was obvious. The statistical significance, and the detection technology were of high specificity and sensitivity. In conclusion, this study adopted double antigen clip combining immune chromatography test EBNA1 antibody in serum, compared with the traditional enzyme-linked immunoassays this method is more rapid, with simpler operation, rapid detection for developing quantum dot immune chromatography technology the EBNA1 antibody kit provides a theoretical basis.
本研究探索了605 nm羧基水溶性量子点,以评估鼻咽癌标志物EBNA1抗体的实用性。我们使用605 nm羧基水溶性量子点和鼻咽癌EBNA1抗原,在1-(3-二甲氨基丙基)-3-乙基碳二亚胺盐酸盐中生成量子点标记抗原。凝胶成像系统显示,30例正常成人血清组和60例鼻咽癌患者血清组亮度有显著差异,两组ELISA吸光度值的Levene检验P<0.001,即鼻咽癌组与正常成人血清组测得的吸光度值差异明显。具有统计学意义,且检测技术具有高特异性和高灵敏度。综上所述,本研究采用双抗原夹心法结合免疫层析检测血清中的EBNA1抗体,与传统酶联免疫法相比,该方法更快速,操作更简单,为开发量子点免疫层析技术检测EBNA1抗体试剂盒提供了理论依据。