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突尼斯北部牛环形泰勒虫和伊氏锥虫的分子流行率及系统发育分析

Molecular prevalence and phylogenetic analysis of Theileria annulata and Trypanosoma evansi in cattle in Northern Tunisia.

作者信息

Sallemi Souhir, Rjeibi Mohamed R, Rouatbi Mariem, Amairia Safa, Ben Said Mourad, Khamassi Khbou Madiha, Gharbi Mohamed

机构信息

Laboratoire de ParasitologieUniv. ManoubaInstitution de la Recherche et de l'Enseignement Supérieur AgricolesÉcole Nationale de Médecine Vétérinaire de Sidi Thabet2020Sidi ThabetTunisia.

出版信息

Vet Med Sci. 2017 Nov 29;4(1):17-25. doi: 10.1002/vms3.79. eCollection 2018 Feb.

DOI:10.1002/vms3.79
PMID:29468077
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5813107/
Abstract

The present study aimed to estimate the molecular prevalence of and infection in cattle in Northern Tunisia. A total number of 96 cattle from five farms were evaluated. and prevalences were 61% [56/66] and 10% [7/13], respectively, at a confidence interval (CI) of 95%, while co-infection was present in 6% [4/8] of the tested animals at a CI of 95%. There was a significant correlation between age and the prevalence of infection, whereas, there was no significant association shown with the age of cattle and infection. Sequence and phylogenetic analyses showed that the Tams1 gene and ITS1 rDNA gene were highly conserved with 97.1-100% and 98.3-100% sequence identity, respectively.

摘要

本研究旨在评估突尼斯北部牛群中[具体病原体名称1]和[具体病原体名称2]感染的分子流行率。对来自五个农场的96头牛进行了评估。在95%置信区间（CI）下，[具体病原体名称1]和[具体病原体名称2]的流行率分别为61%[56/66]和10%[7/13]，而在95%CI下，6%[4/8]的受试动物存在共同感染。年龄与[具体病原体名称1]感染率之间存在显著相关性，然而，未显示牛的年龄与[具体病原体名称2]感染之间存在显著关联。序列和系统发育分析表明，[具体病原体名称1]的Tams1基因和[具体病原体名称2]的ITS1 rDNA基因高度保守，序列同一性分别为97.1 - 100%和98.3 - 100%。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16fc/5813107/93f0ece47488/VMS3-4-17-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16fc/5813107/dbecc7e740d8/VMS3-4-17-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16fc/5813107/9a376ca1e085/VMS3-4-17-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16fc/5813107/a8a6f347ef97/VMS3-4-17-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16fc/5813107/93f0ece47488/VMS3-4-17-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16fc/5813107/dbecc7e740d8/VMS3-4-17-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16fc/5813107/9a376ca1e085/VMS3-4-17-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16fc/5813107/a8a6f347ef97/VMS3-4-17-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16fc/5813107/93f0ece47488/VMS3-4-17-g004.jpg

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