高血压大鼠心脏肥大的进展与消退：心室肌球蛋白的生化及分子变化

Progression and regression of cardiac hypertrophy in hypertensive rats: biochemical and molecular changes in ventricular myosin.

作者信息

Pauletto P, Vescovo G, Scannapieco G, Angelini A, Piccolo D, Pessina A C, Dalla Libera L, Carraro U, Dal Palù C

出版信息

J Hypertens Suppl. 1986 Oct;4(3):S135-7.

Abstract

We investigated the changes in ventricular isomyosin composition and Ca2+-activated ATPase activity during progression and regression of hypertension and cardiac hypertrophy. Eight control male Wistar rats and 14 Goldblatt-II (two-kidney, one clip) hypertensive rats were studied from the 5th week of age. Five of the Goldblatt-II rats underwent nephrectomy of the ischaemic kidney after 5 weeks, with normalization of blood pressure. The hypertensive rats showed a higher biventricular weight to body weight ratio (P less than 0.02), a lower ATPase activity (P less than 0.005), and an increased expression of 'slow' isomyosins in comparison with the age-matched controls. These changes were more pronounced at 15 than at 10 weeks of age. The 15-week-old nephrectomized rats showed the same degree of cardiac hypertrophy, ATPase activity and isomyosin pattern as the age-matched controls. In conclusion, the changes in ventricular myosin observed during the progression of cardiac hypertrophy regressed after normalization of blood pressure and ventricular mass.

摘要

我们研究了高血压和心脏肥大进展及消退过程中心室肌球蛋白组成和钙激活ATP酶活性的变化。从5周龄开始研究8只对照雄性Wistar大鼠和14只Goldblatt-II（双肾，单夹）高血压大鼠。5只Goldblatt-II大鼠在5周后对缺血肾进行了肾切除术，血压恢复正常。与年龄匹配的对照组相比，高血压大鼠的双心室重量与体重比更高（P<0.02），ATP酶活性更低（P<0.005），“慢”肌球蛋白的表达增加。这些变化在15周龄时比10周龄时更明显。15周龄的肾切除大鼠与年龄匹配的对照组表现出相同程度的心脏肥大、ATP酶活性和肌球蛋白模式。总之，在心脏肥大进展过程中观察到的心室肌球蛋白变化在血压和心室质量恢复正常后消退。