使用甲基化特异性PCR研究RB1启动子高甲基化

Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation.

作者信息

McCormick Thaís M, Carvalho Maria Da Glória Da C

机构信息

Laboratory of Molecular Pathology, Department of Pathology, Clementino Fraga Filho University Hospital, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

出版信息

Methods Mol Biol. 2018;1726:29-34. doi: 10.1007/978-1-4939-7565-5_4.

DOI:10.1007/978-1-4939-7565-5_4

PMID:29468541

Abstract

It has increasingly been considered crucial the understanding of DNA methylation of Tumor Suppressor Gene (TSG) promoters, such as that of retinoblastoma 1 gene (RB1), and its role during carcinogenesis. We present a detailed and optimized protocol of the methylation-specific PCR (MSP) technique to study RB1 gene promoter hypermethylation.

摘要

越来越多的人认为，了解肿瘤抑制基因（TSG）启动子的DNA甲基化，如视网膜母细胞瘤1基因（RB1）的DNA甲基化，及其在致癌过程中的作用至关重要。我们提出了一种详细且优化的甲基化特异性PCR（MSP）技术方案，用于研究RB1基因启动子的高甲基化。