Williamson Michael R, John Roseleen F, Colbourne Frederick
Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB, Canada.
Department of Psychology, Neuroscience and Mental Health Institute, University of Alberta, Edmonton, AB, Canada.
Methods Mol Biol. 2018;1717:17-25. doi: 10.1007/978-1-4939-7526-6_2.
Brain injury, such as from stroke and trauma, can be complicated by elevated intracranial pressure (ICP). Although raised ICP can be a significant determinant of morbidity and mortality, clinical studies often report widely varying ICP measurements depending on location of measurement and technique used. For the same reasons, reported ICP measurements also vary widely in animal models. The need for anesthesia or tethered connections with some methods of ICP measurement in animals may introduce additional confounds. Moreover, these methods are not well suited for prolonged, continuous measurement. Here, we describe an approach to continually measure ICP in awake, freely moving rats for several days. This technique uses a commercially available, wireless pressure sensor mounted on the head to measure ICP from the epidural space via a fluid-filled catheter. We have demonstrated that this approach reliably detects elevations in ICP that last for several days after ischemic and hemorrhagic strokes in rat.
脑损伤，如中风和创伤所致，可能会因颅内压（ICP）升高而变得复杂。尽管升高的颅内压可能是发病率和死亡率的重要决定因素，但临床研究常常报告，根据测量位置和所用技术的不同，ICP测量值差异很大。出于同样的原因，在动物模型中报告的ICP测量值也有很大差异。动物进行ICP测量时，因麻醉需求或某些测量方法的固定连接可能会引入额外的混杂因素。此外，这些方法不太适合长时间的连续测量。在此，我们描述一种在清醒、自由活动的大鼠中持续测量ICP数天的方法。该技术使用安装在头部的市售无线压力传感器，通过充满液体的导管从硬膜外腔测量ICP。我们已经证明，这种方法能够可靠地检测大鼠缺血性和出血性中风后持续数天的ICP升高。
