Harishankar Murugesan, Ravikrishnan Harini, Ravishankar Akshaya, Hanna Luke Elizabeth, Swaminathan Soumya, Selvaraj Paramasivam, Bethunaickan Ramalingam
Department of Immunology, National Institute for Research in Tuberculosis, Chennai, India.
Division of HIV, Department of Clinical Research, National Institute for Research in Tuberculosis, Chennai, India.
Curr HIV Res. 2018;16(1):58-63. doi: 10.2174/1570162X16666180219153752.
Genetic factors play an important role in the development of disease susceptibility or protection. Cytokine gene polymorphisms are reported to be associated with altered levels of cytokine production that can impact disease progression in HIV and TB.
In this study, we studied IL-10 -592(C/A) and TGF-β -509 (C/T) promoter polymorphisms to understand their role in susceptibility or resistance to HIV and TB in a South Indian population.
Genomic DNA was isolated from healthy controls, pulmonary tuberculosis patients (n=122) and HIV positive individuals (n=100) and used for genotyping by polymerase chain reaction followed by restriction fragment length polymorphism (PCR-RFLP) method.
Results revealed that under dominant model (CC vs CA+AA), IL-10 -592 'A' allele either 'CA' or 'AA' combinations significantly associated with susceptibility to HIV compared to healthy controls (OR: 1.88(1.05-3.35); p=0.030). However, we found no significant association with TB. TGF-β -509 polymorphism did not associate with either HIV or TB under overdominant model. Neither of the promoter polymorphisms associated with sex in either HIV or TB. However, a trend towards higher risk to HIV was found in females compared with males in IL-10 -592 'AA' genotype.
This study suggests the association of IL-10 -592 "AA" genotype with susceptibility to HIV under dominant model in the Southern Indian population. Future studies are needed with a larger sample size in order to confirm the observations made in this study.
遗传因素在疾病易感性或保护性的发展中起重要作用。据报道，细胞因子基因多态性与细胞因子产生水平的改变有关，这可能影响HIV和结核病的疾病进展。
在本研究中，我们研究了IL-10 -592（C/A）和TGF-β -509（C/T）启动子多态性，以了解它们在印度南部人群对HIV和结核病的易感性或抗性中的作用。
从健康对照、肺结核患者（n=122）和HIV阳性个体（n=100）中分离基因组DNA，并通过聚合酶链反应随后进行限制性片段长度多态性（PCR-RFLP）方法用于基因分型。
结果显示，在显性模型（CC与CA+AA）下，与健康对照相比，IL-10 -592 'A'等位基因（'CA'或'AA'组合）与HIV易感性显著相关（OR：1.88（1.05-3.35）；p=0.030）。然而，我们未发现与结核病有显著关联。在超显性模型下，TGF-β -509多态性与HIV或结核病均无关联。在HIV或结核病中，两种启动子多态性均与性别无关。然而，在IL-10 -592 'AA'基因型中，女性比男性有更高的HIV感染风险趋势。
本研究表明，在印度南部人群的显性模型下，IL-10 -592“AA”基因型与HIV易感性相关。需要进一步开展更大样本量的研究以证实本研究中的观察结果。
