[成虫排泄/分泌蛋白对CLP诱导的小鼠脓毒症的影响]
[Effect of excretory/secretory protein of adult worm on CLP-induced sepsis in mice].
作者信息
Xiao-di Yang, Wen-Xin He, Qiang Fang, Di Song, Qi Wu, Xiao-Li Wang, Nan Li, Qi Qi, Yong-Kun Wan, Hui Zhang, Rui Zhou, Xing-Zhi Chen, Mu-Lin Liu, Hui-Hui Li, Liang Chu
机构信息
Department of Microbiology and Parasitology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China.
Department of Histology and Embryology, Bengbu Medical College, China.
出版信息
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 May 25;28(3):293-296. doi: 10.16250/j.32.1374.2016080.
OBJECTIVE
To observe the effect of excretory/secretory products from adult worms (AES) on cecal ligation and puncture (CLP) -induced sepsis in mice.
METHODS
Forty-eight BALB/c mice were randomly divided into 3 groups：a sham operation group (PBS + sham group, Group A), a CLP-induced sepsis group (PBS+CLP group, Group B) and an AES treatment group (AES+CLP group, Group C). The mice of each group were intraperitoneally injected with 25 μg of AES or PBS only as a control in a total volume of 200 μl. Eight mice from each group were selected randomly for survival analysis of 96 hours. The other 8 mice in each group were observed for pathological changes in the lung, liver and kidney tissues by HE staining 12 h after CLP, and then determined for the detection of cytokines including TNF-α, IL-1β, IL-6, IL-10 and TGF-β in the sera by ELISA.
RESULTS
The difference among the survival rates of mice in the 3 groups was statistically significant ( = 21.16, < 0.05). Compared to Group A (100%), the survival rate of mice in Group B (0) decreased significantly ( < 0.05), and also the pathological damage degrees in the lung, liver and kidney tissues of the mice in Group B increased significantly after CLP. Compared with the mice in group B, the survival rate of those in Group C (70%) increased significantly ( < 0.05), and the pathological damage degrees in the lung, liver and kidney tissues of the mice in Group C decreased significantly after the treatment with AES. The differences among the levels of pro-inflammatory cytokines TNF-α ( = 27.11, < 0.05), IL1β ( = 18.75, < 0.05) and IL-6 ( = 100.93, < 0.05) in the sera of the mice in the three groups were statistically significant. Compared with the mice in Group A, the levels of the 3 cytokines of those in Group B increased significantly (all < 0.05). However, after the treatment with AES, the levels of the pro-inflammatory cytokines of those in Group C decreased significantly (all < 0.05). The differences among the levels of immunoregulatory cytokines IL-10 ( = 10.88, < 0.05) and TGF-β ( = 11.37, < 0.05) in the sera of the mice in the three groups were also statistically significant. Compared with the mice in Group B, the levels of IL-10 and TGF-β of those in Group C were higher after treatment with AES (both < 0.05).
CONCLUSIONS
AES has a therapeutic potential for alleviating sepsis induced by CLP in mice.
目的
观察成虫排泄/分泌产物（AES）对小鼠盲肠结扎穿刺（CLP）诱导的脓毒症的影响。
方法
48只BALB/c小鼠随机分为3组：假手术组（PBS+假手术组，A组）、CLP诱导的脓毒症组（PBS+CLP组，B组）和AES治疗组（AES+CLP组，C组）。每组小鼠腹腔注射25μg AES或仅注射PBS作为对照，总体积200μl。每组随机选取8只小鼠进行96小时的生存分析。每组另外8只小鼠在CLP术后12小时通过HE染色观察肺、肝和肾组织的病理变化，然后通过ELISA法测定血清中细胞因子TNF-α、IL-1β、IL-6、IL-10和TGF-β的含量。
结果
3组小鼠的生存率差异有统计学意义（=21.16，<0.05）。与A组（100%）相比，B组小鼠的生存率（0）显著降低（<0.05），且CLP术后B组小鼠肺、肝和肾组织的病理损伤程度明显增加。与B组小鼠相比，C组小鼠的生存率（70%）显著提高（<0.05），且AES治疗后C组小鼠肺、肝和肾组织的病理损伤程度明显降低。3组小鼠血清中促炎细胞因子TNF-α（=27.11，<0.05）、IL1β（=18.75，<0.05）和IL-6（=100.93，<0.05）水平的差异有统计学意义。与A组小鼠相比，B组小鼠的这3种细胞因子水平显著升高（均<0.05）。然而，AES治疗后，C组小鼠的促炎细胞因子水平显著降低（均<0.05）。3组小鼠血清中免疫调节细胞因子IL-10（=10.88，<0.05）和TGF-β（=11.37，<0.05）水平的差异也有统计学意义。与B组小鼠相比，AES治疗后C组小鼠的IL-10和TGF-β水平更高（均<0.05）。
结论
AES对减轻小鼠CLP诱导的脓毒症具有治疗潜力。
Zotero 插件