[日本血吸虫对吡喹酮的抗药性研究Ⅵ 日本血吸虫吡喹酮抗性株在钉螺体内的生物学特性]

[Studies on resistance of to praziquantel XⅥ Biological characteristics of praziquantel-resistant isolates of in snails].

作者信息

Ke Qian, You-Sheng Liang, Wei Wang, Guo-Li Qu, Hong-Jun Li, Zhen-Kun Yang, Zheng-Yang Zhao, Yuntian Xing, Jian-Rong Dai

机构信息

Key Laboratory of National Health and Family Planning Commission on Technology for Parasitic Disease Prevention and Control, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Public Health Research Center of Jiangnan University, Wuxi 214064, China.

Nanchang Center for Disease Control and Prevention, Jiangxi Province, China.

出版信息

Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2017 Dec 26;29(6):678-682. doi: 10.16250/j.32.1374.2017101.

DOI:10.16250/j.32.1374.2017101

PMID:29469442

Abstract

OBJECTIVE

To investigate the difference of biological characteristics between the praziquantel-resistant and -susceptible isolates of in intermediate host snails.

METHODS

Mice were infected with cercariae of praziquantel-resistant and -susceptible isolates of , and the parasite eggs were collected 37 days post-infection to hatch miracidium. Then, the snails were infected with the miracidium of each parasite isolate. The snail infection, survival rate of infected snails, prepatent period of cercariae, and the total number of cercariae shed from each infected snail were observed and compared between the praziquantel-resistant and -susceptible isolates of .

RESULTS

If each snail was exposed to a single miracidium, there were significant differences between the praziquantel-resistant and -susceptible Jiangsu isolates in the snail infection (8.99% vs. 19.74%; = 3.948, = 0.047) and the number of cercaria released from a single snail (1 460.2 vs. 1 039.3; = 2.507, = 0.02), and there were significant differences between the praziquantel-susceptible and -resistant Hunan isolates in the snail infection (10.00% vs. 21.52%; = 3.980, = 0.046) and the number of cercaria released from a single snail (1 319.4 vs. 1 003.5; = 2.566, = 0.017). However, there were no significant differences between the praziquantel-resistant and -susceptible isolates of in the prepatent period of cercariae and the survival rate of infected snails ( > 0.05).

CONCLUSIONS

The praziquantel-resistant isolate of has a higher susceptibility to but less cercaria released from each infected snail than the susceptible isolate.

摘要

目的

研究日本血吸虫吡喹酮抗性株与敏感株在中间宿主钉螺体内生物学特性的差异。

方法

用日本血吸虫吡喹酮抗性株和敏感株的尾蚴感染小鼠，感染后37天收集虫体虫卵孵化出毛蚴。然后，用各虫株的毛蚴感染钉螺。观察并比较日本血吸虫吡喹酮抗性株与敏感株感染钉螺的情况、感染钉螺的存活率、尾蚴的潜伏期以及每只感染钉螺逸出的尾蚴总数。

结果

若每只钉螺暴露于单个毛蚴，日本血吸虫江苏吡喹酮抗性株与敏感株在钉螺感染率（8.99%对19.74%；χ² = 3.948，P = 0.047）和单个钉螺逸出尾蚴数（1460.2对1039.3；χ² = 2.507，P = 0.02）方面存在显著差异，日本血吸虫湖南吡喹酮敏感株与抗性株在钉螺感染率（10.00%对21.52%；χ² = 3.980，P = 0.046）和单个钉螺逸出尾蚴数（1319.4对1003.5；χ² = 2.566，P = 0.017）方面存在显著差异。然而，日本血吸虫吡喹酮抗性株与敏感株在尾蚴潜伏期和感染钉螺存活率方面无显著差异（P>0.05）。