[]的棒状体蛋白38的原核表达与鉴定
[Prokaryotic expression and identification of rhoptry protein 38 of ].
作者信息
Yong Cui, Jin Li, Hong-Fa Wang, Wei-Xia Zhong, Hui Sun, Gui-Hua Zhao, Kun Yin, Chao Xu, Ting Xiao, Xiao-Yu Zhang, Hong Yu, Xue-Feng Liu, Gong-Zhen Liu
机构信息
Shandong Academy of Medical Sciences, Shandong Institute of Parasitic Diseases, Jining 272033, China.
Animal Husbandry Bureau of Pingyi County.
出版信息
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 May 26;28(5):554-557. doi: 10.16250/j.32.1374.2016060.
OBJECTIVE
To explore the biological function of rhoptry protein 38 (ROP38) of , and to identify the reactogenicity of the recombinant protein (rROP38).
METHODS
The ROP38 was amplified by RT-PCR from RH strain, and was cloned into prokaryotic expression vector pET-28a (+). The recombinant plasmid was transformed into BL21 (DE3) competent cells. Then the rROP38 was analyzed by SDS-PAGE and identified by Western blot.
RESULTS
SDS-PAGE showed that rROP38 was efficient expression with a molecular weight of about 43 kD. Western blot showed that rROP38 reacted with antibody of His tag or human positive antibody, which indicated that ROP38 had good reactogenicity and could be a serological diagnostic antigen.
CONCLUSIONS
The study successfully obtains the rROP38 of with good reactogenicity.
目的
探讨[某种生物]的棒状体蛋白38（ROP38）的生物学功能，并鉴定重组蛋白（rROP38）的反应原性。
方法
通过RT-PCR从RH株中扩增ROP38，并将其克隆到原核表达载体pET-28a(+)中。将重组质粒转化到BL21(DE3)感受态细胞中。然后通过SDS-PAGE分析rROP38，并通过Western blot进行鉴定。
结果
SDS-PAGE显示rROP38高效表达，分子量约为43 kD。Western blot显示rROP38与His标签抗体或人阳性抗体发生反应，表明ROP38具有良好的反应原性，可作为血清学诊断抗原。
结论
本研究成功获得了具有良好反应原性的[某种生物]的rROP38。
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