Ferguson Darren E, Mahan Evan R, Ma Weijun, Bitzas Gabrielle, Zhong Xiaotian, Zollner Richard, D'Antona Aaron M
BioMedicine Design, Medicinal Sciences, Pfizer Global R&D, 610 Main Street, Cambridge, MA, 02139, United States.
BioMedicine Design, Medicinal Sciences, Pfizer Global R&D, 610 Main Street, Cambridge, MA, 02139, United States.
J Chromatogr A. 2018 Mar 23;1542:50-60. doi: 10.1016/j.chroma.2018.02.018. Epub 2018 Feb 12.
Advancement in high-throughput screening methods of novel therapeutic proteins for early stage research and development, specifically mAbs, have given mid-scale (milligram to gram scale) purification groups access to more of these molecules. The available purification technologies built to support mid-scale production was not efficient or versatile enough to keep up with this surge. To remedy this problem, we have designed and built a custom instrument using an ÄKTA Pure. This system enables parallel processing up to 5 samples and has the versatility to perform 1- to 3-step purification processes in a single queue. Furthermore, a unique purification scheme can be selected for each of the five samples in the queue. Overall processing time has reduced by 83% compared to manual, non-parallel load methods. Here, we describe our novel approach and demonstrate the flexibility, speed and efficiency of the instrument.
