Wang Xueqin, Yu Huahua, Xing Ronge, Chen Xiaolin, Liu Song, Li Pengcheng
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
PeerJ. 2018 Feb 15;6:e4373. doi: 10.7717/peerj.4373. eCollection 2018.
Mackerel () viscera contain large amount of protein. We used five proteases to hydrolyze the viscera, and the hydrolysate treated by neutrase exhibited the highest nitrogen recovery (NR). Then we optimized the preparation conditions for mackerel viscera hydrolysate (MVH) by response surface methodology and investigated the antioxidant activity of MVH. The optimal conditions were as follows: enzyme concentration of 1,762.87 U/g, pH of 6.76, temperature of 43.75 °C, extraction time of 6.0 h and water/material ratio of 20.37 (v/w), and the maximum NR was 37.84%. Furthermore, the molecular weight distribution of MVH was almost below 3,000 Da determined by TSK G2000 SWXL gel filtration chromatography, and the MVH exhibited good antioxidant activities in various assays, including DPPH radical, hydroxyl radical and superoxide anion scavenging activities, reducing power and similar effectivelness as butylated hydroxytoluene and Vitamin E to inhibit lipid peroxidation. The results suggested that MVH could be used as a potential source of antioxidant peptide in food industries.
鲭鱼（）内脏含有大量蛋白质。我们使用了五种蛋白酶来水解内脏，其中经中性蛋白酶处理的水解产物表现出最高的氮回收率（NR）。然后我们通过响应面法优化了鲭鱼内脏水解物（MVH）的制备条件，并研究了MVH的抗氧化活性。最佳条件如下：酶浓度为1762.87 U/g，pH为6.76，温度为43.75℃，提取时间为6.0小时，水/物料比为20.37（v/w），最大氮回收率为37.84%。此外，通过TSK G2000 SWXL凝胶过滤色谱法测定，MVH的分子量分布几乎都低于3000 Da，并且MVH在各种测定中表现出良好的抗氧化活性，包括DPPH自由基、羟基自由基和超氧阴离子清除活性、还原能力以及与丁基羟基甲苯和维生素E抑制脂质过氧化的效果相似。结果表明，MVH可作为食品工业中潜在的抗氧化肽来源。
