需要组装：汤姆·史蒂文斯实验室对 V-ATP 酶领域的贡献。
Some assembly required: Contributions of Tom Stevens' lab to the V-ATPase field.
机构信息
Institute of Molecular Biology, University of Oregon, Eugene, Oregon.
Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, Kansas.
出版信息
Traffic. 2018 Jun;19(6):385-390. doi: 10.1111/tra.12559. Epub 2018 Mar 25.
Tom Stevens' lab has explored the subunit composition and assembly of the yeast V-ATPase for more than 30 years. Early studies helped establish yeast as the predominant model system for study of V-ATPase proton pumps and led to the discovery of protein splicing of the V-ATPase catalytic subunit. The Vma phenotype, characteristic of loss-of-V-ATPase activity in yeast was key in determining the enzyme's subunit composition via yeast genetics. V-ATPase subunit composition proved to be highly conserved among eukaryotes. Genetic screens for new vma mutants led to identification of a set of dedicated V-ATPase assembly factors and helped unravel the complex pathways for V-ATPase assembly. In later years, exploration of the evolutionary history of several V-ATPase subunits provided new information about the enzyme's structure and function. This review highlights V-ATPase work in the Stevens' lab between 1987 and 2017.
汤姆·史蒂文斯（Tom Stevens）的实验室已经研究酵母 V-ATPase 的亚基组成和组装超过 30 年。早期的研究有助于确立酵母成为研究 V-ATPase 质子泵的主要模型系统，并导致 V-ATPase 催化亚基的蛋白质剪接的发现。酵母中 V-ATPase 活性丧失的 Vma 表型对于通过酵母遗传学确定该酶的亚基组成至关重要。V-ATPase 亚基组成在真核生物中高度保守。用于鉴定新的 vma 突变体的遗传筛选导致了一组专门的 V-ATPase 组装因子的鉴定，并有助于阐明 V-ATPase 组装的复杂途径。在后来的几年中，对几种 V-ATPase 亚基的进化历史的探索提供了有关该酶结构和功能的新信息。这篇综述重点介绍了史蒂文斯实验室在 1987 年至 2017 年间在 V-ATPase 方面的工作。
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