聚合酶链反应多重检测在前段葡萄膜炎患者巨细胞病毒感染中的应用。
PCR Multiplex for CMV Detection in Patients with Anterior Uveitis.
机构信息
a Department of Microbiology and Parasitology , Hospital Universitario de la Princesa , Madrid , Spain.
b Department of Ophthalmology , Hospital Universitario de la Princesa , Madrid , Spain.
出版信息
Ocul Immunol Inflamm. 2019;27(2):197-202. doi: 10.1080/09273948.2018.1438633. Epub 2018 Feb 23.
PURPOSE
Describing the utility of Polymerase Chain Reaction for Cytomegalovirus (CMV-PCR) in the diagnosis of suspected viral anterior uveitis (AU).
PATIENTS AND METHODS
We analyzed aqueous humor (AH) CMV-PCR positivity and treatment modifications in 47 eyes of 46 patients with viral uveitis and its correlation with high intraocular pressure (HIOP), uveitis clinical characteristics and time intervals from samples to uveitis diagnosis and to relapse.
RESULTS
CMV-PCR positive results occurred in 13 eyes (27.7%) of 12 patients. They were more frequent in HIOP eyes (34.2%, p = 0.047) and with Posner-Schlossman Syndrome (56.2%, p = 0.002). CMV-PCR positivity (p = 0.001) and HIOP (p = 0.038) increased the probability of treatment change. Although CMV-PCR positive results decreased over time (p = 0.002), they were not related to activity or proximity to inflammatory uveitis episode.
CONCLUSION
HIOP AU eyes should be considered for CMV-PCR AH analysis due to possible treatment modifications.
目的
描述聚合酶链反应（PCR）在巨细胞病毒（CMV-PCR）诊断疑似病毒性前葡萄膜炎（AU）中的应用。
方法
我们分析了 46 例病毒性葡萄膜炎患者的 47 只眼的房水 CMV-PCR 阳性率和治疗改变情况，并将其与高眼压（HIOP）、葡萄膜炎临床特征以及从样本到葡萄膜炎诊断和复发的时间间隔进行相关性分析。
结果
12 例患者的 13 只眼（27.7%）CMV-PCR 阳性结果。HIOP 眼（34.2%，p=0.047）和 Posner-Schlossman 综合征（56.2%，p=0.002）的阳性率更高。CMV-PCR 阳性（p=0.001）和 HIOP（p=0.038）增加了治疗改变的可能性。尽管 CMV-PCR 阳性结果随时间减少（p=0.002），但与炎症性葡萄膜炎发作的活动度或接近度无关。
结论
由于可能的治疗改变，HIOP AU 眼应考虑进行 CMV-PCR 房水分析。
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