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人类环杓后肌(PCA)与膈肌:作为再支配尝试基础的组织化学比较

The human posterior cricoarytenoid (PCA) muscle and diaphragm. A histochemical comparison as a basis for reinnervation attempts.

作者信息

Brøndbo K, Dahl H A, Teig E, Gujord K M

出版信息

Acta Otolaryngol. 1986 Nov-Dec;102(5-6):474-81. doi: 10.3109/00016488609119433.

DOI:10.3109/00016488609119433
PMID:2947418
Abstract

PCA (posterior cricoarytenoid) muscles and biopsies from the SCM (sterno-cleidomastoid) muscles as well as the diaphragm were serially sectioned and incubated for myofibrillar ATPase and selected metabolic enzymes. The three main fibre types were present in all muscles, although some PCA muscles seemed to lack IIB fibres. The mean fibre type pattern of the PCA muscle was 57% type I, 36% type IIA and 7% type IIB, as compared with 42% type I, 42% type IIA and 16% type IIB in the diaphragm. All fibre types of the PCA muscle and the diaphragm were significantly more oxidative and less glycolytic than the corresponding SCM muscle fibres. Most striking was the finding of high 3-HBDH activity in the PCA and diaphragm muscle fibres, especially in type I.

摘要

对环杓后肌(PCA)、胸锁乳突肌(SCM)以及膈肌进行活检,将这些肌肉进行连续切片,并针对肌原纤维ATP酶和选定的代谢酶进行孵育。所有肌肉中均存在三种主要的纤维类型,尽管一些环杓后肌似乎缺乏IIB型纤维。环杓后肌的平均纤维类型模式为57% I型、36% IIA型和7% IIB型,而膈肌中相应的比例为42% I型、42% IIA型和16% IIB型。与胸锁乳突肌的相应纤维相比,环杓后肌和膈肌的所有纤维类型均具有显著更高的氧化能力和更低的糖酵解能力。最显著的发现是环杓后肌和膈肌纤维中3-羟丁酸脱氢酶(3-HBDH)活性较高,尤其是I型纤维。

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